Mass spectrometry for comparative proteomics of degenerative and regenerative processes in the brain
Full text
Related documents
Key words: Alzheimer’s disease, cerebrospinal fluid, frontotemporal dementia, neurodegeneration, proteomics, mass spectrometry, prefractionation, protein
The aim of this thesis was to develop methods that can be used to quantify endogenous peptides and proteins in cerebrospinal fluid (CSF) to identify potential biomarkers for AD..
Proteomic and mass spectrometry approaches were used to characterize the composition of the human colonic mucus layer in health an disease, and to determine how alterations in protein
Taken together, the results from this thesis show that the human colonic mucus is composed of a relatively small number of proteins that are organized around the
The aim of this thesis was to exploit these features by developing methods for the analysis of endogenous steroids, cholesterol oxidation products (COPs) and thyroid hormones (THs) by
When combining the classical biomarkers with MS-based markers the diagnostic accuracy of distin- guishing AD (including MCI/AD converters) from patients with other cognitive
Electrospray Ionisation 4.1 General principle of electrospray 4.2 The role of ESI in analytical chemistry 4.3 Electrospray development history 4.4 ESI ionisation process 4.4.1
The aim of this thesis was to investigate the use of alternative MS-based techniques to assist specific analytical challenges including separation of stereoisomers using