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Errata
This errata lists the errors and corrections for the doctoral thesis of Ivana Cengic, titled
“Synthetic biology approaches for improving production of fatty acid-derived
compounds in cyanobacteria” ISBN: 978-91-7873-178-7.The line numbering relates to the number of lines of body text on the indicated page.
Page I, line 3-4. The section "…produced chemical…” …produced chemicals…”.
Page II, keywords. The keyword “alkane deformylating oxygenase” should read “aldehyde deformylating oxygenase”.
Page IV, line 23. A period should be added after “...kolväten.
Chairman has been changed. Should be:
Prof. Stefan Ståhl
Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, Stockholm, Sweden.
Page 1, line 20. The section “…the costs of…” should read “…the cost of…”.
Page 3, line 16. The organism name “Synechocystis elongatus PCC 7942” should read
“Synechococcus elongatus PCC 7942”.
Page 3, line 17. The section “…model organism…” should read “…model organisms…”.
Page 4, Figure 1. The metabolic pathways should in addition feature arrowheads pointing from 3PGA to G3P, as well as from G3P to F6P.
Page 9, Figure 2. The naming “pre-crRNA-trarcRNA-Cas9 complex” should read “pre-crRNA- tracrRNA-Cas9 complex”.
Page 10, line 5. The section “…crRNA-tracrRNA complxs…” should read “…a pre-cRNA- tracrRNA complex…”.
Page 10, line 5-6. The sentences “An RNase III endogenous to the host processes this into a mature crRNAs. The resulting mature crRNA-tracrRNA duplex directs Cas9 to its target by binding to the complementary protospacer, and the nuclease domains in Cas9 catalyze the double stranded break (DSB). “ should read "An RNase III endogenous to the host processes this into mature crRNA-tracrRNA duplexes that direct Cas9 to its targets by binding to the complementary protospacers, whereby the nuclease domains in Cas9 catalyze the double stranded break (DSB).
Page 11, line 8. A comma should be removed in the section “…including, doubling…”.
Page 13, line 20. The sentence "The Synechocystis study showed that E. coli antigen 43 was able
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to translocate correctly in the non-native cyanobacteria, and allow display of its own antigen 43 passenger domain, which was susceptible to degradation by externally added proteinase K.”should read “Here, the E. coli antigen 43 was able to translocate correctly in the non-native cyanobacteria and allow display of its own antigen 43 passenger domain, which was susceptible to degradation by externally added proteinase K.”
Page 14, line 21. The section “...protein, defined as type I FAS…” should read “...protein complex, defined as type I FAS…”.
Page 12, line 23. The section “…using several discrete enzymes…” should read “…using discrete enzymes for each pathway step…”.
Page 15, Figure 4. The arrowhead for the reaction catalysed by LPLAT should point in the other direction, i.e. the LPLAT consumes an acyl-ACP.
Page 16, line 12. The section “…have shown …” should read “…has shown…”.
Page 16, line 16. The section “…to produced…” should read “…to produce …”.
Page 16, line 18. The section “…the β-hydroxyacyl-ACP intermediate…” should read “…the β- hydroxyacyl-ACP FAS-intermediate…”.
Page 17, line 17. The section “…using exogenous…” should read “…use exogenous…”.
Page 17, line 18. The section “…mechanism currently known…” should read “…mechanisms currently known…”.
Page 19, line 2. The section “...diffuse through the…” should read “...diffuse throughout the…”.
Page 20, line 28. The section “…fatty aldehyde reductase…” should read “…fatty aldehyde reductase (AHR)…”.
Page 21, Figure 5. The placement of some of the enzyme names are incorrect, the corrected version is as follows:
fatty acyl-ACP
R S-ACP
O
R OH
O
fatty acid
fatty acyl-CoA
R S-CoA
O
R H
O
fatty aldehyde
R OH
fatty alcohol
β-oxidation pathway FASII
TES CAR
AL-FAR AL-FAR
AHR
FadD
OH-FAR OH-FAR
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Page 22, line 1. The section “…synthases…” should read “…synthases (e.g. E. coli FadD)…”.Page 24, line 1. The section “…acetyl-CoA reductase…” should read “…acyl-CoA reductase…”.
Page 24, line 22-23. The section “However, in vitro characterization of Ado has revealed it…”
should read “However overall, in vitro characterization of Ado has also revealed it…”.
Page 24, line 36. The number in “O2” should be subscript, i.e. “O2".
Page 26, line 11. The section “…N. punctiforme…” should read “...Nostoc puntiforme…”.
Page 26, line 22. The section “...Nostoc puntiforme…” should read “…N. punctiforme…”.
Page 29, line 9. The section “…domain in…” should read “…domain on…”.
Page 29, line 18. The section “…candidate carrier proteins.” should read “…candidate display systems.”
Page 31, line 10. The section “Analysis of the Slp fusion proteins…” should read “Analysis of the strains expressing Slp fusion proteins…”.
Page 33, line 19. The section “…deactivated (dCas9)…” should read “…deactivated Cas9 (dCas9)…”.
Page 36, line 14. The section “…to work done on the DK01 strains.” should read “…to work done on the DK01 strain.”.
Page 38, figure text for Figure 15. “Synechocystis” should be in italics, i.e. “Synechocystis”.
Page 39, line 10. The section “…level of membrane alteration (Fig. 16D).” should read “…level of membrane alteration in fatty alcohol producing strains (Fig. 16D).”.
Page 40, line 13. The section “…pathway.” should read “…pathway (Fig. 17).”.
Page 40, line 14. The section “…affecting transcriptional regulation…” should read “…effecting transcriptional regulation…”.
Page 40, line 20. The section “…cause causes build-up…” should read “…cause build-up…”.
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Page 43, Figure 21A. The datasets of dCas9 and sgRNA4 have incorrectly been assigned the same color. See below for a corrected version.Page 44, line 17. The section “…and reaction …” should read “…and reacting…”.
Page 44, Figure 22A. The growth rates on the y-axis in the figure are incorrect. See below for a corrected version.
Page 45, line 10. The section “…engineer strains…” should read “…engineering strains …”.
Page 45, line 22-23. The strain name “S. elongatus” should read “S. elongatus PCC 7942".
Page 47, Figure 24B. The legend for the red bars should read “C17:1-ene”.
Page 48, line 17. The section “…help elucidating…” should read “…help in elucidating …”.
Page 49, line 29. The section “…fatty acid-derived products…” should read “…fatty acid- derived products in cyanobacteria…”.
0 1 2 3 4
0 2 4 6 8 10
Time (days) OD730
dCas9 sgRNA1 sgRNA2
sgRNA3 sgRNA4
