Anotace:
j ena
metody LIFT v minulosti -
Annotation
Bachelor thesis is focused on the preparation of biosensors by means of laser methods, laser induction of forward transmission (abbreviated LIFT). In this paper is a brief introduction mainly to LASER, thin layers and transfer methods themselves. Furthermore, the use of LIFT methods using past tests and Matrix-Assisted Pulsed Laser Evaporation methods is demonstrated. The findings are furthermore used in practice for transferring gold as a good conductor to a slide which acts as an insulator.
Keywords: LIFT, laser, biosensor, thin film
, Ph.D. za pomoc , a za
.
10
Seznam zkratek ... 12
... 13
1 Lasery ... 14
1.1 Princip ... 14
1.2 ... 15
1.3 ... 15
2 ... 17
3 Metoda Laser Induced Forward Transfer ... 19
3.1 Historie metody Laser Induced Forward Transfer ... 19
3.2 Princip metody Laser Induced Forward Transfer ... 19
3.3 Experimenty ... 21
3.3.1 ... 21
3.3.2 ... 22
3.3.2.1 Biomolekuly ... 22
3.3.2.2 Roztok DNA ... 22
3.3.3 ... 23
3.3.3.1 Biomolekuly a liposomy ... 23
3.3.3.2 DNA vzory ... 25
4 Matrix-Assisted Pulsed Laser Evaporation ... 26
5 ... 27
5.1 ... 27
5.2 ... 27
5.3 ... 27
5.4 ... 29
5.4.1 ... 29
11
5.4.2 ... 31
5.5 ... 34
5.6 ... 36
5.6.1 ... 36
5.6.2 ... 37
5.6.3 ... 39
Diskuze ... 42
... 44
... 445
... 448
... 449
12
Seznam zkratek
Apod.
Atd.
CW Continual wave
CD Compact disc
DNA
HiLASE High average power pulsed LASErs
LASER Light amplification by stimulated emission radiation LIFT Laser induceed forward transfer
LW Laser writening
MAPLE Matrix-Assisted Pulsed Laser Evaporation Mj.
MTR Material laser recordings Obr.
SDS
SW Software
TP Triazen polymer
UV Ultraviolet
13
na
e bude
, resp. metoda LIFT, byla a jako jedna z
krv k Je to velmi
diagnozy
biosenzoru s Vzhledem
. velk elektrick vodivost
4,5.107 S/m [1], a s jeho 0,0235 [2]. P
sklo bylo zvoleno vy, je izolantem,
. Ta
elektrick vodivost 4.102 S/m [3] a
rezistivitu 3.103 [3]. Z
, n
14
1 Lasery
Slovo LASER je zkratka z Light Amplification by Stimulated Emission of Radiation
K to
[4]
1.1 Princip laseru
element
ntum
inverze populace).
Teprve v
e
k [4]
15
1.2
resp.
[4]
1.3
elektricky, radio- N
a CO2 [4]
je Nd:YAG
[5]
no
-
- prvky jako zrcadla
10
16
[5]
[5]
Excimer lasery
[5]
-
a- ultrafast -
-
17
2 vrstvy
-
a
k
[6]
[7]
18
ten
- vodivost - odolnost -
-
- polarizace - permitivita
- koeficient -
-
- tvrdost
- elasticita
-
- rovinnost - mikrostruktura -
- index lomu - absorpce -
- -
- odolnost proti korozi a erozi
. [8]
3 Metoda Laser Induced Forward Transfer
3.1 Historie metody Laser Induced Forward Transfer
zkr. LIFT z angl. Laser Induced Forward Transfer),
. [9]
z z ang. material laser
a nazval jej laser induced forward transfer.
. [10]
(typ laseru XeCl), 351 nm (typ laseru XeF). [13]
3.2 Princip metody Laser Induced Forward Transfer
ho
m filmu, n
filmem (viz 1 a 2). [11]
[12]
z
stavu), ale v
20
biomolekul. [14]
. [14]
1: Ilustrace principu metody LIFT [16]
2: [15]
21
[16]
3.3 Experimenty
3.3.1 v proces
(XeCl 308nm, ArF 193 nm, Nd:
deskami.
Paprsek tak
vzorku.
(1 mm nebo 2 mm). [17]
22
3.3.2
3.3.2.1 Biomolekuly
biomolekul
2 mg/ml. [17] Tento roztok
a manipulace s tenkou vrstvou kapaliny s obsahem biomolekul.
-
SDS), st roztoku n . [18]
3.3.2.2 Roztok DNA
PBS. Pelet byl znovu
[17]
Potom se k DNA
Supernatant byl
[11]
23
3.3.3
3.3.3.1 Biomolekuly a liposomy
jak pro typy laseru ArF, a XeCl
liposomy. Pro tyto experimenty,
nebyly
s typem laseru XeCl, svazek
TP 60 nm. V
( 3).
( 4).
s . [17]
24
3: [17]
4:
kapaliny [17]
25
3.3.3.2 DNA vzory
l
400 um. roztoku
roztoku DNA s ohledem na interakci laser->
energie
v
hloubka, to el na
I o to
na citlivosti
s obsahem DNA v kontaktu s kovovou vrstvou. Kapalina se vlivem tepla a je
na ho o
roztoku
roztokem DNA. [17]
26
4 Matrix-Assisted Pulsed Laser Evaporation
T -Assisted Pulsed Laser Evaporation (zkr. MAPLE) byla
vyvinuta a
pro polymery.
. V MAPLE
a aby je, a
M
. Jakmile jsou molekuly na
vy V MAPLE chlaz
. % (m/m)
ro u ( )
energie je typicky nastavena mezi 0,01 a 1,0 J/cm2
.
, t
[19]
27
5
5.1
V na jednom
byly provedeny i pokusy s
, s jedn
em s nap
-li kdyby bylo to linek.
5.2 Metodika
centru HiLASE (High average power pulsed LASErs) mezi
a
bylo
rozhodnuto adhez
pro zlat .
5.3
S z fakulty Praze.
od firmy JEOL, t.
k
h Petriho
, prachu
28
. -
.
( 5). V
( 6). . N
.
5: k [20]
6: u [20]
29
5.4 lasery
Pro tvorbu typu Omron MX-AI2030
TruMark 3000. energie , modelu
TruMark 3 tento
.
5.4.1
-AI2030 series
.
v
lo pokusech
se aplikoval pouze jeden pulz (single shot).
V
a
stolek ( 7, 8, str. 30 a 9 str. 31).
v
azku
30
se
7: laser, 2 [20]
8: [20]
31 9:
[20]
5.4.2
bylo rozhodnuto zkusit
ku filtry. Pro
svazek, 10, str. 32
( 11, str. 32).
v TruMark
32
10: Laser TruMark 3000 [20]
11: [20]
33
(obr 12, obr 13) svazku
ho u ve svazku B).
12: zek [20]
13: Svazek ( ) [20]
34
5.5
M
tzv
e C), kam
v
konfiguraci soustavy tomto
software
ose x,y. kon
0mA a na 2000mA (viz Graf 1)
.
[20]
, v . Laser
typu Omron MX-AI2030
35
a to by vedlo k
laseru -
expozice. ylo
se jeden bylo o
y ,
se zlatou vrstvou a
stolku v
(v ohnisku , a
, a tak
).
doby byla
tj. bez mezery mezi nimi. V bylo vyzkou mezery
mezi ( 14)
Ale bylo tato
mik v .
14: [20]
36
5.6
Na mikroskop Olympus OLS 4000. Tento mikroskop
disponuje mimo
5.6.1
latou vrstvou v
a to v ohnisku , a i
od 700mA do 1700mA.
zlatou
vrstv . Pokud k a
vrstvy i v
( 15) (1700mA)
k ho ,
proudech 1900-2000mA).
15: 750mA) [20]
37
16: [20]
5.6.2
Jak
v ale . energii
z
kontaktu
16
vrstvou a ach neus
mezerou i bez
38
17: 1600mA v ohnisku
[20]
19 (str. 39
18:
[20]
39
19: 1900mA [20]
5.6.3
e spolu s
vlastnosti -li skl .
a
z
svazku proudu okolo 1750mA
posunem stolku mimo ohnisko
svazku zlata
( )
40
20: 1750mA, vzorek mimo
ohnisko ( )[20]
.
Na 21,
.
21:
[20]
41
s ku, kdy vzorek
svazek byl
j - . Spolu
s
a obrazce na
zlatou vrstvu skoro v 22) na .
,
soustavy proud na 2000mA
se
zlata tohoto kruhu
22:
[20]
42
Diskuze
byla snaha
Z mate
by d z bodu na
linku apod.) . Na strukturu by vrstva na jednom
Oproti
. T
do prostoru v mikro .
pokusech (viz kapitola 3)
schopni zlatou
proudu laserovou diodou v
a ohniska .
, je nevhodn ,
hodnot. Jedna z
,
zlata .
43
hledat chybu i v
pokusech nebyl v
v ,
obal mohly ovlivnit.
Jedno z bylo eno
sam s laserem
toto byly ny
Vzhledem k
pouze
s ), ale v
vlastnosti by se nejednalo
44
V byla snaha
T
podle
vrstvy k mj. masky
v azku
sebou, k
bylo opakov . S
bylo potvrzeno ,
byl .
tato
se s
k ne siln
u sebe u . (
P lze
s
tomuto nedojde.
45
2009.
http://www.prvky.com/79.html
2 . 3. vyd.
ISBN 80-85849-84-4.
z: http://www.prvky.com/14.html
4 Miroslav. -PLD,
MAPLE .
5 - -
Lao Lasery a Optika [online]. Praha, [cit. 2017-04- http://www.lao.cz/lao-info-49/serial-na-tema-lasery---zakladni-princip- laseru-a-jejich-deleni-127
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7 . Brno, 2014.
https://www.vutbr.cz/www_base/zav_prace_soubor_verejne.php?file_id=85267
8
elektrotechnice. Vyd. 4. Praha: BEN , 2006. ISBN 978-80-7300-1.
46
9 GONG, Hanmo a kol. Gold nanoparticle transfer through photothermal effects in a metamaterial absorber by nanosecond laser, Scientific Reports. 2014,4, 6080. ISSN 2045- 2322.
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http://www.sciencedirect.com/science/article/pii/0169433294003858
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nanoscale, transparent, and solid-phase materials
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17 PALLA-PAPAVLU, Alexandra a kol. Laser induced forward transfer for materials patterning, Romanian Reports in Physics. 2011, 63(Supplement), 1285-1301. ISSN 1841-
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www.sciencedirect.com/science/article/pii/S0040609003016432#
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20 Archiv Autora
-1.
met
48
Ilustrace principu metody LIFT (12)
kapaliny
laser, 2
Laser TruMark 3000
zek 13:
mimo
49
- -1 ed 3.
-
- Norma ISO 14644-1
-1 ed 3.
23 [20]
24 o [20]
- Norma ISO 14644-1
Norma ISO 14644-
[22]
parametry jako teplota, vlhkost a tlak.
ou,
Klasifikace
J
ISO [22]
klasifikace ISO (N)
ISO 1 10 2
ISO 2 100 24 10 4
ISO 3 1000 237 102 35 8
ISO 4 10000 2370 1020 352 83
ISO 5 100000 23700 10200 3520 832 29
ISO 6 1000000 237000 102000 35200 8320 293
ISO 7 352000 83200 2930
ISO 8 3520000 832000 29300
ISO 9 35200000 8320000 293000
[22]