GA 101003666 Start date: 01/04/20 End Date: 31/03/22
Project Title OPENCORONA
WP number,
deliverable number, and Title
WP 2 D2.2 : Assessment of cytokine induction and IFN suppression by first set of vaccine candidates
Responsible partner
name and contact Partner number: 2 Organisation: JLU
Name: Friedemann Weber
Email: friedemann.weber@vetmed.uni-giessen.de Nature
R-Report P-Prototype D-Demonstrator O=-Other
R
Dissemination level PU-public
PP-restricted to otherprogramme participants
RE-restricted to a group of partners CO-only for
consortium members
PU (to be published)
Delivery Month
Planned 6
Actual delivery date
(dd/mm/yy) 14/10/2020
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 101003666
1 Ref. Ares(2020)6591228 - 11/11/2020
Description of deliverable
• COMPLETED
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 101003666
D 2.2: Assessment of cytokine induction and IFN suppression by first set of vaccine candidate.
1. RT-qPCR analyses of the first set of vaccine candidates (cytokine inductions, A549 cells)
1 10 100 1000 10000 100000 1000000
IFN-β mRNA
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ISG15 mRNA
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IFN-λ1 mRNA
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MxA mRNA
ü slight activation of IFNs and ISGs ü very little cytokine activation
ü inhibition of type I (but not type III) induction and signalling by OC-11 (M alone)
2
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 101003666
3
1 10 100
IL-10 mRNA
1 10 100
TNF-α mRNA
1 10 100
IL-8 mRNA
1 10 100 1000
IL-6 mRNA
2. Reporter assays to investigate activation of Tol-like receptors (TLR 2, 3, 4, 7, 8, 9)
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 101003666
4
ü Systems have been set up for TLRs 2,3,4,7,8,9 ü No activation of any TLR, not even TLR4