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The Interaction of Candidalysin and Human Neutrophils

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Scientific project, 30 hp

Biomedical Laboratory Science Programme, 180 hp Autumn 2017

The Interaction of Candidalysin and

Human Neutrophils

(2)

Department of Clinical Microbiology Biomedical Laboratory Science programme

Scientific project, 30 hp

Course coordinator: Ylva Hedberg Fransson

ylva.hedberg.fransson@umu.se

Opponent:

Mari Norgren

Examiner:

Ylva Hedberg Fransson

Date of pass:

2018 02 19

Supervisor

(3)

Abstract

Fungal infections are a major cause of human disease and mortality worldwide. The fungi Candida

albicans is an innocuous member of the human microbiome, but is also known to cause life threatening

invasive infections, especially in immune-compromised individuals. Human neutrophils build the first line of defence regarding fungal infections, either by engulfing the microorganism or releasing neutrophil extracellular traps (NET). C. albicans hyphae are known to trigger NET formation. Recently, a new peptide toxin, candidalysin, was identified in C. albicans hyphae, which can lyse multiple cell types. The aim of the study was to investigate the interaction between candidalysin and human neutrophils, and to investigate which signalling mechanisms in neutrophils were triggered by candidalysin to release NET. In conclusion, it was demonstrated that candidalysin was able to induce NET formation within 120 min, and the ideal reaction time was 240 to 300 min. Additional results indicated that candidalysin most likely influenced a pathway that involved Syk, PI3K, AKT, mTOR, and NF-κB to trigger neutrophils to release NET. However, these findings require further investigation.

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