Supplemental Figure S1. Comparisson of our microarray data with previous studies using venn diagrams. log2FC values in red denotes oposite trend in expression.

(1)

A. Overlap between genes regulated by solar UV A+B in Ler with UV-B regulated genes in wild-type plants (Brown et al., 2005 and Favory et al., 2009)

Ler UV AB Genes regulated by solar UV A+B in Ler after 12 h

Col UVB 6h↓ Genes with decreased expression in Col, UV-B 1.5 µmol m-2 s-1 for 6 h (Favory et al., 2009) Col UVB 6h↑ Genes with increased expression in Col, UV-B 1.5 µmol m-2 s-1 for 6 h (Favory et al., 2009) Ler UVB Genes induced in Ler, UV-B 3 µmol m-2 s-1 for 4 h (Brown et al., 2005)

A1. Ler UV ABvs Col UVB 6h↑ Symbol log2FC in our study

1 At5g07990 TT7 3,388 2 At4g04840 MSRB6 2,091 3 At1g65560 F5I14.9 1,551 4 At1g54570 T22H22.2 1,623 5 At5g48880 PKT2 1,733 6 At2g37040 PAL1 1,678

(2)

7 At1g06690 F12K11.2 1,080 8 At3g19170 PREP1 0,873 9 At1g36160 ACC1 1,233 10 At3g53260 PAL2 1,412 11 At1g48850 EMB1144 0,792 12 At5g51040 K3K7.22 0,770 13 At1g10522 … 0,831 14 At1g48570 T1N15.19 0,740 15 At1g73990 SPPA 1,101 16 At4g32770 VTE1 0,663 17 At1g12050 F12F1.8 0,710 18 At3g49320 F2K15.180 0,685 19 At3g57180 F15A17.240 0,636 20 At5g48470 MJE7.11 0,745

A2. Ler UV ABvs Col UVB 6h↑ vsLer UVB

1 At5g62210 MMI9.4 2,212 2 At3g22840 ELIP1 1,678 3 At5g13930 CHS 1,412 4 At4g31870 GPX7 1,519 5 At4g14690 ELIP2 2,952 6 At5g60540 PDX2 1,286 7 At1g78570 RHM1 1,225 8 At5g08640 FLS1 1,470 9 At5g05270 K18I23.7 1,446 10 At3g55120 CHI 1,566 11 At5g17050 UGT78D2 0,999 12 At5g02270 ABCI20 0,900 13 At3g51240 F3H 1,952 14 At5g24850 CRY3 0,851

(3)

15 At5g23730 RUP 2 0,943

16 At1g06000 UGT89C1 0,953

17 At5g56090 COX15 0,863

18 At3g16530 MDC8.19 -1,490

19 At4g37760 SQE3 0,635

A3. Ler UV ABvsLer UVB

1 At5g42800 DFR 1,197 2 At2g18690 MSF3.7 -1,375 3 At1g56060 T6H22.17 -1,660 4 At5g27420 CNI1 -1,412 5 At2g29350 SAG13 -0,844 6 At3g14620 CYP72A8 -1,009 7 At3g55980 SZF1 -1,016 8 At2g38470 WRKY33 -1,420 9 At2g02010 GAD4 -0,705 10 At4g27280 M4I22.90 -1,608 11 At2g18680 MSF3.6 -1,142

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B. Overlap between candidate UVR8 regulated genes under solar UV A+B with UVR8 regulated genes under UV-B (Brown et al., 2005)

uvr8-2 UVAB UVR8 regulated genes by solar UV A+B 12 h

UVR8 UV-B UVR8 regulated genes, UV-B 3 µmol m-2 s-1 for 4 h (Brown et al., 2005)

1 At5g24120 SIG5 1,222 2 At5g02270 ABCI20 0,643 3 At3g56290 F18O21.250 0,694 4 At5g42800 DFR -1,697 5 At1g78510 SPS 0,803 6 At3g17800 MEB5.2 0,922

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C. Overlap between candidate UVR8 regulated genes under solar UV A+B with UVR8 regulated genes under UV-B (Favory et al., 2009)

uvr8-2 UVAB UVR8 regulated genes by solar UV A+B 12 h

UVR8 1 h Genes not induced in uvr8-6 after 1 h UV-B (1.5 µmol m-2 s-1) UVR8 6 h Genes not induced in uvr8-6 after 6 h UV-B (1.5 µmol m-2 s-1) UVR8 96 h Genes not induced in uvr8-6 after 96 h UV-B (1.5 µmol m-2 s-1)

C1. uvr8-2 UVAB vs UVR8 1 h

1 At5g19240 T24G5.140 -1,107

2 At3g17800 MEB5.2 0,922

3 At1g28480 GRX480 -0,844

4 At3g05800 AIF1 0,741

C2. uvr8-2 UVAB vs UVR8 6 h

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2 At1g69730 T6C23.7 -0,692

3 At5g01600 FER1 1,308

4 At3g56090 FER3 0,652

C3. uvr8-2 UVAB vs UVR8 1 h vsUVR8 6 h

1 At5g24120 SIG5 1,222 2 At5g18470 T28N17.6 -1,099 3 At1g78510 SPS 0,803 4 At1g19180 JAZ1 -0,795 5 At4g26530 M3E9.40 0,708 6 At3g56290 F18O21.250 0,694 7 At5g02270 ABCI20 0,643 8 At1g67070 PMI2 0,756

D. Overlap between wild type and uvr8-2 gene lists under UV A+B 12 h with COP1 regulated genes under UV-B and white light (Oravecz et al., 2006)

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COP1 UVB COP1 regulated genes under UVB COP1 WL COP1 regulated genes under white light

Ler UV AB Genes regulated by solar UV A+B in Ler after 12 h

D1. uvr8 UV AB vs COP1 UVB

1 At1g28480 GRX480 -0,844 2 At1g78510 SPS 0,803 3 At1g67070 PMI2 0,756 D2. uvr8 UV AB vs COP1 WL 1 At3g28220 T19D11.1 -1,463 2 At5g19240 T24G5.140 -1,107 3 At1g19180 JAZ1 -0,795

D3. uvr8 UV AB vs COP1 UVB vsCOP1 WL

1 At5g24120 SIG5 1,222

2 At3g17800 MEB5.2 0,922

3 At3g56290 F18O21.250 0,694

4 At5g01520 F7A740 0,949

D4. Ler UV AB vs COP1 UVB

1 At4g14690 ELIP2 2,952 2 At3g55980 SZF1 -1,016 3 At4g24570 DIC2 -1,619 4 At4g31870 GPX7 1,519 5 At5g17050 UGT78D2 0,999 6 At5g24850 CRY3 0,851 7 At5g62210 MMI9.4 2,212 8 At5g23730 RUP 2 0,943 9 At5g05270 K18I23.7 1,446 10 At5g60540 PDX2 1,286 11 At5g56090 COX15 0,863

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12 At3g53260 PAL2 1,412 13 At1g65560 F5I14.9 1,551 14 At5g08640 FLS1 1,470 15 At3g55120 CHI 1,566 16 At4g32770 VTE1 0,663 17 At1g73990 SPPA 1,101 18 At1g06000 UGT89C1 0,953 19 At2g37040 PAL1 1,678 20 At5g13930 CHS 1,412 21 At1g06690 F12K11.2 1,080 D5. Ler UV AB vs COP1 WL 1 At4g37760 SQE3 0,635

D6. Ler UV AB vs COP1 UVB vsCOP1 WL

1 At3g22840 ELIP1 1,678

2 At3g51240 F3H 1,952

D7. Ler UV AB vs COP1 UVB vsuvr8 UV AB

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E. Overlap between wild type and uvr8-2 gene lists under UV A+B 12 h with blue light regulated genes (Kleine et al., 2007)

uvr8-2 UVAB UVR8 regulated genes by solar UV A+B 12 h Ler UV AB Genes regulated by solar UV A+B in Ler after 12 h

Blue light Blue light regulated genes, 3 h (200 µmol photons m-2 s-1)

E1. Ler UV AB vs Blue light

1 At3g22840 ELIP1 1,678 2 At3g51240 F3H 1,952 3 At5g62210 MMI9.4 2,212 4 At5g23730 RUP 2 0,943 5 At5g17050 UGT78D2 0,999 6 At5g05270 K18I23.7 1,446 7 At4g37760 SQE3 0,635 8 At5g08640 FLS1 1,470 9 At5g07990 TT7 3,388 10 At1g65560 F5I14.9 1,551

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11 At4g04840 MSRB6 2,091 12 At4g32770 VTE1 0,663 13 At5g13930 CHS 1,412 14 At4g24570 DIC2 -1,619 15 At1g56060 T6H22.17 -1,660 16 At1g78570 RHM1 1,225 17 At3g14620 CYP72A8 -1,009 18 At3g11930 MEC18.3 0,816 19 At1g13650 F21F23.9 0,819 20 At1g19960 T20H2.25 -0,813 21 At5g48880 PKT2 1,733 22 At4g31870 GPX7 1,519 23 At2g37040 PAL1 1,678 24 At4g14690 ELIP2 2,952 25 At5g60540 PDX2 1,286 26 At1g06690 F12K11.2 1,080 27 At3g55120 CHI 1,566 28 At5g24850 CRY3 0,851 29 At1g06000 UGT89C1 0,953 30 At3g53260 PAL2 1,412

E2. uvr8 UV AB vs Blue light

1 At1g56650 PAP1 -1,484 2 At5g24120 SIG5 1,222 3 At3g56290 F18O21.250 0,694 4 At3g05800 AIF1 0,741 5 At1g78510 SPS 0,803 6 At3g29590 AT5MAT -1,397 7 At3g17800 MEB5.2 0,922 8 At5g01600 FER1 1,308 9 At4g30650 F17I23.10 -0,662

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10 At3g56090 FER3 0,652 11 At5g54060 UF3GT -1,529 12 At5g47370 HAT2 -2,976 13 At1g31550 T8E3.19 -0,970 14 At2g14560 LURP1 -0,868 15 At1g19670 CLH1 -1,505 16 At2g28400 T1B3.8 -1,076 17 At1g16850 F6I1.15 -0,848

E3. Ler UV AB vs uvr8 UV AB vs Blue light

1 At5g02270 ABCI20

2 At5g42800 DFR

F. Overlap between wild type and uvr8-2 gene lists under UV A+B 12 h with high light regulated genes (Kleine et al., 2007)

CRY1 High light regulated genes CRY1 dependent Ler UV AB Genes regulated by solar UV A+B in Ler after 12 h uvr8 UV AB UVR8 regulated genes by solar UV A+B 12 h

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F1. Ler UV AB vs CRY1 vs HY5 1 At4g14690 ELIP2 2,952 2 At4g31870 GPX7 1,519 3 At5g60540 PDX2 1,286 4 At5g24850 CRY3 0,851 5 At5g56090 COX15 0,863 6 At1g06000 UGT89C1 0,953 7 At2g37040 PAL1 1,678 8 At3g55120 CHI 1,566 9 At5g48880 PKT2 1,733 F2. Ler UV AB vs CRY1 1 At3g22840 ELIP1 1,678 2 At5g62210 MMI9.4 2,212 3 At5g08640 FLS1 1,470 4 At3g51240 F3H 1,952 F3. uvr82 UV AB vs HY5 1 At2g28400 T1B3.8 -1,076 2 At1g16850 F6I1.15 -0,848 3 At3g56400 WRKY70 -0,700 F4. uvr82 UV AB vs CRY1 1 At1g78510 SPS 0,803 2 At1g56650 PAP1 -1,484

F4. Ler UV AB vs uvr82 UV AB vs CRY1

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Supplemental Figure S2. (A) Pictures of wild-type (Ler) and uvr8-2 plants germinated and grown for three weeks outdoors under the UV treatments. Scale bars = 1 cm. (B) Average number of flowering plants per pot.

Ler

uvr8-2

UV A+B

UV A

UV 0

A

B

0 1 2 3 4 5 6 UV 0 UV A UV A+B Ler uvr8-2

p

N

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m

b

e

r

o

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la

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(

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Supplemental Figure S3. Spectral irradiance (W m-2 nm-1) under the UV treatments

measured with a spectroradiometer Maya2000 Pro during a sunny day at noon.

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Supplemental Table S1. Genes regualted by UV in wild-type Ler after 12 h Log2FC(log2 fold change)

P (P-value adjusted for multiple testing)

No Gene symbol AGI No log2FC P Description

1 MMI9.4 At5g62210 2,212 0,001 Embryo-specific protein 3, (ATS3)

2 TT7 At5g07990 3,388 0,001 Required for flavonoid 3' hydroxylase activity

3 MSRB6 At4g04840 2,091 0,001 Methionine sulfoxide reductase domain

4 ELIP1 At3g22840 1,678 0,003 Encodes an early light-inducible protein

5 F5I14.9 At1g65560 1,551 0,004 Zinc-binding dehydrogenase family protein 6 T22H22.2 At1g54570 1,623 0,004 Esterase/lipase/thioesterase family protein

7 CHS At5g13930 1,412 0,005 Encodes chalcone synthase (CHS)

8 LDOX At4g22880 1,383 0,005 Encodes leucoanthocyanidin dioxygenase

9 PKT2 At5g48880 1,733 0,005 Peroxisomal 3-keto-acyl-CoA thiolase 2 precursor

10 GPX7 At4g31870 1,519 0,005 Encodes glutathione peroxidase

11 PAL1 At2g37040 1,678 0,005 Encodes phenylalanine ammonia-lyase

12 GSTF2 At4g02520 -1,711 0,008 Glutathione transferase, phi class of GSTs

13 ELIP2 At4g14690 2,952 0,008 Encodes an early light-induced protein

14 DFR At5g42800 1,197 0,008 Encodes dihydroflavonol reductase

15 DIC2 At4g24570 -1,619 0,008 Mitochondrial dicarboxylate carriers (DIC)

16 F14P13.5 At3g10350 1,115 0,008 Anion-transporting ATPase family protein

17 PDX2 At5g60540 1,286 0,008 Involved in vitamin B6 biosynthesis

18 F12K11.2 At1g06690 1,080 0,008 NAD(P)-linked oxidoreductase superfamily protein

19 MSF3.7 At2g18690 -1,375 0,011 Unknown protein

20 RHM1 At1g78570 1,225 0,013 Involved in the biosynthesis of rhamnose

21 FLS1 At5g08640 1,470 0,021 Encodes a flavonol synthase

22 PCR2 At1g14870 -0,940 0,022 Encodes plant cadmium resistance 2 (PCR2) 23 K18I23.7 At5g05270 1,446 0,023 chalcone-flavanone isomerase family protein

24 CHI At3g55120 1,566 0,023 Catalyzes conversion of chalcones into flavanones

25 UGT78D2 At5g17050 0,999 0,024 Encodes a anthocyanidin 3-O-glucosyltransferase

26 ABCI20 At5g02270 0,900 0,024 ATP-BINDING CASSETTE I20 (ABCI20) 27 PREP1 At3g19170 0,873 0,025 Zinc metalloprotease pitrilysin subfamily A

28 T6H22.17 At1g56060 -1,660 0,025 Unknown protein

29 CNI1 At5g27420 -1,412 0,025 Encodes CNI1 (Carbon/Nitrogen Insensitive1)

30 F3H At3g51240 1,952 0,025 Encodes flavanone 3-hydroxylase

31 ACC1 At1g36160 1,233 0,025 Encodes acetyl-CoA carboxylase

32 F7H19.50 At4g22870 0,882 0,025 oxygenase superfamily protein

33 PAL2 At3g53260 1,412 0,026 Encodes phenylalanine ammonia-lyase

34 MPK3 At3g45640 -1,145 0,029 Response to UV-B and oxidative stress

35 … At5g26690 -1,177 0,029 Heavy-metal-associated domain-containing protein

36 SAG13 At2g29350 -0,844 0,029 Senescence-associated gene SAG13

37 EMB1144 At1g48850 0,792 0,029 Embryo defective 1144 (EMB1144)

38 CYP72A8 At3g14620 -1,009 0,029 Putative cytochrome P450

39 K3K7.22 At5g51040 0,770 0,032 Unknown protein

40 ARL At2g44080 -1,163 0,032 Cell expansion, brassinosteroid signaling pathway

41 SZF1 At3g55980 -1,016 0,032 Salt inducible finger 1, transcription factor activity

42 ERD13 At2g30870 -0,965 0,032 Early dehydration-induced gene

43 PRB1 At2g14580 -2,009 0,029 Ethlylene, jasmonic acid and salycilic acid stimulus

44 T20H2.25 At1g19960 -0,813 0,034 Unknown protein

45 CRY3 At5g24850 0,851 0,034 Cryptochrome 3

46 … At1g10522 0,831 0,035 Unknown protein

47 ALD1 At2g13810 -1,488 0,037 AGD2-Like defense response protein 1 (ALD1)

48 FAMT At3g44860 -0,792 0,037 farnesoic acid carboxyl-O-methyltransferase

49 F1E22.7 At1g65690 -0,913 0,037 Late embryogenesis abundant (LEA) 50 F21F23.9 At1g13650 0,819 0,037 Unknown protein

51 PCR1 At1g14880 -0,875 0,037 Plant Cadmium resistance 1 (PCR1) 52 F3N23.11 At1g72910 -0,706 0,037 Defense response, signal transduction

53 T9J22.6 At2g26390 -0,712 0,037 Serine protease inhibitor (SERPIN) family protein 54 F18O19.11 At2g43780 0,726 0,037 Unknown protein

55 T1N15.19 At1g48570 0,740 0,037 Zinc finger (Ran-binding) family protein

56 MYM9.4 At3g23700 0,868 0,037 Nucleic acid-binding proteins superfamily 57 SPPA At1g73990 1,101 0,039 Encodes a putative protease SppA (SppA).

58 ISA3 At4g09020 -0,948 0,039 Encodes an isoamylase-like protein

59 WRKY33 At2g38470 -1,420 0,043 Member of the plant WRKY transcription factor family

60 F13O11.3 At1g64710 -0,962 0,045 GroES-like zinc-binding dehydrogenase family protein 61 F24M12.370 At3g51330 -0,822 0,045 Eukaryotic aspartyl protease family protein

62 RUP 2 At5g23730 0,943 0,045 Transducin/WD40 repeat-like superfamily protein 63 RD22 At5g25610 -0,850 0,045 Responsive to dehydration mediated by ABA

64 GRP-3 At2g05520 -0,688 0,045 Encodes a glycine-rich protein

65 PR1 At2g14610 -1,898 0,045 Pathogenesis related gene

66 GAD4 At2g02010 -0,705 0,045 Glutamate decarboxylase 4 (GAD4)

67 VTE1 At4g32770 0,663 0,045 Tocopherol cyclase

68 GSTF8 At2g47730 -0,651 0,045 Encodes glutathione transferase

69 CSLG2 At4g24000 -1,605 0,045 Encodes a protein similar to cellulose synthase

70 T16L1.250 At4g33760 0,644 0,045 tRNA synthetase class II (D, K and N) family protein

71 UGT89C1 At1g06000 0,953 0,045 Encodes a flavonol-7-O-rhamnosyltransferase

72 COX15 At5g56090 0,863 0,045 Cytochrome C Oxidase 15 73 F12F1.8 At1g12050 0,710 0,045 Fumarylacetoacetase putative

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74 K20I9.8 At3g16850 -0,659 0,046 Pectin lyase-like superfamily protein 75 ACYB-2 At4g25570 0,798 0,047 Encodes cytochrome b561

76 MDC8.19 At3g16530 -1,490 0,048 Lectin like protein

77 MSN2.11 At5g66720 0,649 0,048 Protein phosphatase 2C family protein 78 PME41 At4g02330 -0,780 0,048 Brassinosteroid stimulus, response to cold 79 T31J12.3 At1g09310 -0,765 0,048 Unknown protein

80 SQE3 At4g37760 0,635 0,049 Squalene epoxidase 3 (SQE3)

81 F2K15.180 At3g49320 0,685 0,049 Metal-dependent protein hydrolase

82 MEC18.3 At3g11930 0,816 0,049 Adenine nucleotide alpha hydrolases-like protein 83 M4I22.90 At4g27280 -1,608 0,050 Calcium-binding EF-hand family protein

84 NADP-ME2 At5g11670 -0,813 0,049 Encodes NADP-MALIC enzyme 2

85 MHJ24.1 At5g64170 -0,636 0,050 Dentin sialophosphoprotein-related

86 GLX2-5 At2g31350 -0,620 0,050 Encodes a mitochondrial glyoxalase 2

87 MSF3.6 At2g18680 -1,142 0,050 Unknown protein

88 TOM5 At5g08040 0,616 0,050 Mitochondrial import receptor subunit TOM5 homolog 89 BG3 At3g57240 -1,388 0,050 Encodes a member of glycosyl hydrolase family 17 90 MNB8.3 At5g52970 0,643 0,050 Thylakoid lumen 15.0 kDa protein

91 F15A17.240 At5g03210 -0,870 0,050 Unknown protein

92 F15A17.240 At3g57180 0,636 0,050 Brassinazole Insensitive Pale Green 2 93 T20F21.27 At2g35710 -0,912 0,053 Nucleotide-diphospho-sugar transferase 94 MJE7.11 At5g48470 0,745 0,050 Unknown protein

95 MZN24.8 At3g21940 0,633 0,050 Receptor protein kinase-related 96 SEX4 At5g46230 -0,660 0,050 Protein phosphatase

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Supplemental Table S2. Genes regualted by UV in uvr8-2 after 12h LogFC(log2 fold change)

P (P-value adjusted for multiple testing)

No Gene symbol AGI No logFC P Description

1 CLH1 At1g19670 -1,505 0,02 Chlorophyll degradation 2 T19D11.1 At3g28220 -1,463 0,02 TRAF-like family protein

3 LDOX At4g22880 -1,700 0,02 Encodes leucoanthocyanidin dioxygenase 4 T1B3.8 At2g28400 -1,076 0,02 Protein of unknown function

5 WAK1 At1g21250 -1,016 0,02 Cell wall-associated kinase, involved in signaling and SA stimulus 6 SIG5 At5g24120 1,222 0,02 Sigma factor, response to blue, red and far red light

7 DFR At5g42800 -1,697 0,02 Encodes dihydroflavonol reductase

8 T24G5.140 At5g19240 -1,107 0,02 Glycoprotein membrane precursor GPI-anchored 9 F12E4.80 At5g03350 -1,322 0,02 Legume lectin family protein

10 AOS At5g42650 -1,059 0,02 p450 CYP74 gene family that functions as an allene oxide synthase 11 T28N17.6 At5g18470 -1,099 0,02 Curculin-like (mannose-binding) lectin family protein

12 ANNAT1 At1g35720 -0,997 0,02 Encodes a member of the annexin gene family, response to stress 13 ATR4 At4g31500 -0,965 0,02 Involved in the biosynthetic pathway of glucosinolates

14 ANK At5g54610 -1,228 0,03 Belongs to the ankyrin repeat protein family, response to SA stimulus 15 XPL1 At3g18000 0,844 0,03 N-methyltransferase-like protein, lipid biosynthesis and reproduction 16 RLK At2g37710 -0,943 0,03 Receptor lectin kinase, phosphorilation, response to SA stimulus 17 MEB5.2 At3g17800 0,922 0,03 Response to UV-B

18 T1P17.80 At4g12490 -0,918 0,03 defence response to fungus

19 GRX480 At1g28480 -0,844 0,04 Glutaredoxin family, involved in SA/JA signaling pathways 20 AT5MAT At3g29590 -1,397 0,04 anthocyanidin 5-O-glucoside-6"-O-malonyltransferase 21 LHT7 At4g35180 -0,832 0,04 Lys/His transporter 7

22 MXO21.9 At3g29240 -0,813 0,04 Protein of unknown function 23 T6J4.20 At1g13470 -0,964 0,04 Protein of unknown function

24 SPS At1g78510 0,803 0,04 Solanesyl diphosphate synthase activity

25 FAMT At3g44860 -0,873 0,04 Encodes farnesoic acid carboxyl-O-methyltransferase

26 F7H19.50 At4g22870 -1,388 0,04 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase protein 27 F4L23.31 At2g45180 0,941 0,04 Bifunctional inhibitor/lipid-transfer protein

28 F14D7.1 At1g35710 -1,127 0,04 Protein kinase family protein with leucine-rich repeat domain 29 LURP1 At2g14560 -0,868 0,04 Response to Hyaloperonospora parasitica

30 T22A6.310 At4g24480 3,502 0,04 Protein kinase

31 GRP19 At5g07550 3,452 0,04 Glycine rich protein 19, lipid storage and sexual reproduction 32 F12K8.24 At1g22410 -0,721 0,05 Class-II DAHP synthetase family protein

33 AOC1 At3g25760 -0,776 0,05 Encodes allene oxide cyclase, jasmonic acid biosynthesis 34 AGP21 At1g55330 1,021 0,05 Encodes a putative arabinogalactan-protein (AGP21)

35 JAZ1 At1g19180 -0,795 0,05 Jasmonate-Zim-Domain protein 1, involved in defence response 36 M3E9.40 At4g26530 0,708 0,05 Aldolase superfamily protein

37 AOC3 At3g25780 -0,997 0,05 Involved in jasmonic acid biosynthesis

38 PPa5 At4g01480 -0,777 0,05 Encodes a protein that might have inorganic pyrophosphatase activity 39 XBAT34 At4g14365 -0,990 0,05 Zinc ion binding protein

40 T16B24.15 At2g39210 -0,768 0,05 Major facilitator superfamily protein

41 UF3GT At5g54060 -1,529 0,05 UDP-glucose:flavonoid 3-o-glucosyltransferase 42 T8E3.19 At1g31550 -0,970 0,05 GDSL-like Lipase/Acylhydrolase superfamily protein 43 F21A20.190 At5g27480 -3,179 0,05 transposable element gene

44 HAT2 At5g47370 -2,976 0,05 Auxin mediated signaling pathway 45 DL4760C At4g17450 2,927 0,05 Transposable element gene

46 ERD13 At2g30870 -0,697 0,05 Early dehydration-induced gene , response to water deprivation 47 SYR1 At3g11820 -0,780 0,05 Defence response, JA mediating signaling pathway

48 T3K9.5 At2g41180 -0,882 0,05 Protein of unknown function

49 SIB1 At3g56710 -0,856 0,05 SIGMA FACTOR BINDING PROTEIN 1 (SIB1) 50 F15K19.1, At2g10940 0,783 0,05 Bifunctional inhibitor/lipid-transfer protein 51 F6I1.15 At1g16850 -0,848 0,05 Response to salt stress

52 T3K9.14 At2g41090 -0,765 0,05 Calcium-binding EF-hand family protein 53 F21M11.2 At1g04040 0,872 0,05 HAD superfamily

54 DL4760C At4g17450 2,927 0,05 transposable element gene 55 F6H5.8 At3g32220 2,788 0,06 Gypsy-like retrotransposon family

56 HPL1 At4g15440 -1,085 0,06 HYDROPEROXIDE LYASE 1 (HPL1), response to wounding 57 AIF1 At3g05800 0,741 0,06 Brassinosteroid mediated signaling pathway

58 MES5 At5g10300 -0,746 0,06 METHYL ESTERASE 5 (MES5)

59 PAP1 At1g56650 -1,484 0,06 PRODUCTION OF ANTHOCYANIN PIGMENT 1 (PAP1)

60 WRKY70 At3g56400 -0,700 0,06 WRKY DNA-BINDING PROTEIN 70 (WRKY70), defence response 61 F18O21.250 At3g56290 0,694 0,06 Protein of unknown function

62 F7A740 At5g01520 0,949 0,06 RING/U-box superfamily protein involved in zinc ion binding 63 T24G5.150 At5g19250 -0,630 0,06 Protein of unknown function

64 F17I23.10 At4g30650 -0,662 0,06 Low temperature and salt responsive protein, defence response 65 ABCI20 At5g02270 0,643 0,06 ATP-BINDING CASSETTE I20 (ABCI20)

66 At5g26690 -0,833 0,06 Heavy metal transport/detoxification superfamily protein 67 MKK4 At1g51660 -0,636 0,06 Mitogen-activated map kinase, defence response

68 SOT17 At1g18590 -0,615 0,06 SULFOTRANSFERASE 17, glucosinolate core structure biosynthesis 69 F6N7.24 At5g52750 -0,836 0,06 Heavy metal transport/detoxification superfamily protein

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70 T6C23.7 At1g69730 -0,692 0,06 Wall-associated kinase family protein

71 T32A17.160 At4g08850 -0,830 0,06 Leucine-rich repeat receptor-like protein kinase family protein 72 F28O16.9 At1g76720 0,652 0,06 eukaryotic translation initiation factor 2 (eIF-2) family protein 73 F6H5.8 At3g32220 2,788 0,06 transposable element gene

74 F12F1.28 At1g11880 2,457 0,07 GPI anchor biosynthetic process, lipid biosynthetic processes 75 LCR27 At4g29300 2,393 0,07 Protein of unknown function

76 F1M20.12 At1g74440 -0,642 0,07 Protein of unknown function

77 AGP31 At1g28290 0,935 0,07 ARABINOGALACTAN PROTEIN 31, response to JA stimulus 78 FER1 At5g01600 1,308 0,07 FERRETIN 1 (FER1), response to ROS

79 FER3 At3g56090 0,652 0,07 FERRITIN 3 (FER3), response to ROS 80 UCP5 At2g22500 -0,660 0,07 Uncoupling protein 5

81 IGMT5 At1g76790 -0,906 0,07 INDOLE GLUCOSINOLATE O-METHYLTRANSFERASE 5 (IGMT5) 82 JR1 At3g16470 -0,758 0,07 JASMONATE RESPONSIVE 1 (JR1), response to abiotic stress 83 PMI2 At1g67070 0,756 0,07 L-ascorbic acid biosynthesis

84 BTS At3g18290 -0,631 0,07 E3 ligase protein with metal ion binding and DNA binding domains 85 PRXCB At3g49120 -0,674 0,07 PEROXIDASE CB, defence response to bacteria and fungus 86 ATPCB At3g49120 -0,674 0,07 Defence response to bacterium and fungus

87 OPR3 At2g06050 -0,799 0,07 Jasmonic acid biosynthesis process 88 T7N9.9 At1g27030 -0,749 0,07 Protein of unknown function

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Supplemental Table S3. Detailed information on experiments represented in the Bayesian cluster (Fig. 1)

Experiment Annotation Treatment UV-BBE Reference

Col-0 MeJA 6 h EATMX-13 50 µM methyl jasmonate 1

Col-0 MeJA 30 min EATMX-13 50 µM methyl jasmonate 1

Col-0 MeJA 2 h EATMX-13 50 µM methyl jasmonate 1

Col-0 ABA 30 min NASCARRAYS-176 10 µM abscisic acid 30 min 7

Col-0 ABA 1 h NASCARRAYS-176 10 µM abscisic acid 1 h 7

cop1-4 305 nm 15 min E-MEXP-557 Broadband UVB (305 nm cut-off) 0.12 Wm2 2 WS 305 nm 6 h E-MEXP-550 Broadband UVB (305 nm cut-off) 0.12 Wm2 3 WS 295 nm 6 h E-MEXP-550 Broadband UVB (295 nm cut-off) 0.42 Wm2 3 uvr8-6 305 nm 1 h E-MEXP-1957 Narrowband UVB (305 nm cut-off) 1.5 µmol m-2 s-1 4 Col-0 unfiltered UV lamps 15 min NASCARRAYS-137 Broadband UVB 15 min 1.18 Wm2 NA uvr8-6 305 nm 6h E-MEXP-1957 Narrowband UVB (305 nm cut-off) 1.5 µmol m-2 s-1 4

Col-0 SA 24 h GSE14961 2mM salicylic acid 24 h NA

Col-0 Ethylene 4 h GSE14247 Ethylene gas 10 ppm 5

Col-0 BTH 8 h NASCARRAYS-392 60 µM BTH 8 h NA

WS unfiltered UV lamps 6 h E-MEXP-550 Broadband UVB (unfiltered) 1.18 Wm2 3 Col-0 unfiltered UV lamps 15 min harvested 3 h NASCARRAYS-144 Broadband UVB 15 min 1.18 Wm2 6 Col-0 unfiltered UV lamps 15 min harvested 6 h NASCARRAYS-144 Broadband UVB 15 min 1.18 Wm2 6 Ler 305 nm 15 min E-MEXP-557 Narrowband UVB (305 nm cut-off) 0.12 Wm2 2 Col-0 305 nm 15 min E-MEXP-557 Narrowband UVB (305 nm cut-off) 0.12 Wm2 2 WS 305 nm 1 h E-MEXP-550 Broadband UVB (305 nm cut-off) 0.12 Wm2 3 Col-0 305 nm 1 h E-MEXP-1957 Narrowband UVB (305 nm cut-off) 1.5 µmol m-2 s-1 4 Col-0 305 nm 6 h E-MEXP-1957 Narrowband UVB (305 nm cut-off) 1.5 µmol m-2 s-1 4 hy5 305 nm 15 min E-MEXP-557 Broadband UVB (305 nm cut-off) 0.12 Wm2 2 WS unfiltered UV lamps 1 h E-MEXP-550 Broadband UVB (unfiltered) 1.18 Wm2 3 WS 295 nm 1 h E-MEXP-550 Broadband UVB (295 nm cut-off) 0.42 Wm2 3 Col-0 unfiltered UV lamps 15 min harvested 1 h NASCARRAYS-144 Broadband UVB 15 min 1.18 Wm2 6 Col-0 unfiltered UV lamps 15 min harvested 30 min NASCARRAYS-144 Broadband UVB 15 min 1.18 Wm2 6

Col-0 ABA 3 h NASCARRAYS-176 10 µM abscisic acid 3 h 7

Col-0 SA 3 h NASCARRAYS-192 10 µM salicylic acid 3 h NA

Col-0 unfiltered UV lamps 15 min harvested 12 h NASCARRAYS-144 Broadband UVB 15 min 1.18 Wm2 6 Col-0 unfiltered UV lamps 15 min harvested 24 h NASCARRAYS-144 Broadband UVB 15 min 1.18 Wm2 6

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Reference

Pauwels et al (2008) Proc Natl Acad Sci USA 105: 1380-1385 1 Oravecz et al (2006) Plant Cell 18: 1975–1990 2 Ulm et al (2004) Proc Natl Acad Sci USA 101: 1397–1402 3

Favory et al (2009) EMBO J 28: 591–601 4

Qiao et al (2009) Genes Dev 23: 512-21 5

Kilian et al (2007) Plant J 50 :347-363 6

Goda et al (2008) Plant J 55: 526-542 7

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Supplemental Table S4. Detailed information on genes represented in the Bayesian cluster (Fig. 1).

Genes shown in Fig. 1 as they appear from left to right. The fold change induction in wild-type and uvr8-2

with the corresponding P value and the cluster they group are also given

Wild-type

uvr8-2

No

AGI code

LogFc

P

LogFc

P

Cluster

1 At5g64170

-0,64

0,05

I

2 At5g62210

2,21

0,001

I

3 At5g60540

1,29

0,01

I

4 At5g54060

-1,53

0,05

I

5 At5g52970

0,64

0,05

I

6 At5g51040

0,77

0,03

I

7 At5g48880

1,73

0,01

I

8 At5g48470

0,74

0,05

I

9 At5g42800

1,20

0,01

-1,70

0,02

I

10 At5g42650

-1,06

0,02

I

11 At5g25610

-0,85

0,04

I

12 At5g24850

0,85

0,03

I

13 At5g23730

0,94

0,04

I

14 At5g19250

-0,63

0,06

I

15 At5g17050

1,00

0,02

I

16 At5g13930

1,41

0,005

I

17 At5g11670

-0,81

0,05

I

18 At5g08640

1,47

0,02

I

19 At5g08040

0,62

0,05

I

20 At5g07990

3,39

0,001

I

21 At5g07550

3,45

0,04

I

22 At5g05270

1,45

0,02

I

23 At5g03210

-0,87

0,05

I

24 At5g02270

0,90

0,02

0,64

0,06

I

25 At5g01600

1,31

0,07

I

26 At5g01520

0,95

0,06

I

27 At4g37760

0,63

0,05

I

28 At4g33760

0,64

0,04

I

29 At4g32770

0,66

0,04

I

30 At4g30650

-0,66

0,06

I

31 At4g26530

0,71

0,05

I

32 At4g25570

0,80

0,05

I

33 At4g24570

-1,62

0,01

I

34 At4g24480

3,50

0,04

I

35 At4g24000

-1,61

0,04

I

36 At4g15440

-1,08

0,06

I

37 At4g12490

-0,92

0,03

I

38 At4g09020

-0,95

0,04

I

39 At4g04840

2,09

0,001

I

40 At4g02330

-0,78

0,05

I

41 At4g01480

-0,78

0,05

I

42 At3g57180

0,64

0,05

I

43 At3g56290

0,69

0,06

I

44 At3g56090

0,65

0,07

I

45 At3g55120

1,57

0,02

I

46 At3g53260

1,41

0,03

I

47 At3g52180

-0,66

0,05

I

48 At3g51330

-0,82

0,04

I

49 At3g49320

0,68

0,05

I

50 At3g45640

-1,14

0,03

I

51 At3g32220

2,79

0,06

I

52 At3g29590

-1,40

0,04

I

53 At3g29240

-0,81

0,04

I

54 At3g23700

0,87

0,04

I

55 At3g19170

0,87

0,02

I

56 At3g18290

-0,63

0,07

I

57 At3g18000

0,84

0,03

I

58 At3g17800

0,92

0,03

I

59 At3g16850

-0,66

0,05

I

60 At3g16470

-0,76

0,07

I

61 At3g11930

0,82

0,05

I

62 At3g10350

1,11

0,01

I

63 At3g05800

0,74

0,06

I

64 At2g45180

0,94

0,04

I

65 At2g44080

-1,16

0,03

I

66 At2g43780

0,73

0,04

I

67 At2g41090

-0,77

0,05

I

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68 At2g35710

-0,91

0,05

I

69 At2g31350

-0,62

0,05

I

70 At2g30870

-0,96

0,03

-0,70

0,05

I

71 At2g26390

-0,71

0,04

I

72 At2g14580

-2,01

0,03

I

73 At2g13810

-1,49

0,04

I

74 At2g10940

0,78

0,05

I

75 At2g05520

-0,69

0,04

I

76 At1g78570

1,23

0,01

I

77 At1g78510

0,80

0,04

I

78 At1g76790

-0,91

0,07

I

79 At1g74440

-0,64

0,07

I

80 At1g73990

1,10

0,04

I

81 At1g69730

-0,69

0,06

I

82 At1g67070

0,76

0,07

I

83 At1g64710

-0,96

0,04

I

84 At1g56650

-1,48

0,06

I

85 At1g55330

1,02

0,05

I

86 At1g54570

1,62

0,00

I

87 At1g48850

0,79

0,03

I

88 At1g48570

0,74

0,04

I

89 At1g36160

1,23

0,03

I

90 At1g35720

-1,00

0,02

I

91 At1g35710

-1,13

0,04

I

92 At1g31550

-0,97

0,05

I

93 At1g28290

0,93

0,07

I

94 At1g22410

-0,72

0,05

I

95 At1g19670

-1,51

0,02

I

96 At1g18590

-0,61

0,06

I

97 At1g16850

-0,85

0,05

I

98 At1g13650

0,82

0,04

I

99 At1g12050

0,71

0,05

I

100 At1g10522

0,83

0,04

I

101 At1g09310

-0,76

0,05

I

102 At1g06690

1,08

0,01

I

103 At1g04040

0,87

0,05

I

104 At1g06000

0,95

0,04

I

105 At5g56090

0,86

0,05

II

106 At5g54610

-1,23

0,03

II

107 At5g52750

-0,84

0,06

II

108 At5g27420

-1,41

0,02

II

109 At5g24120

1,22

0,02

II

110 At5g19240

-1,11

0,02

II

111 At5g18470

-1,10

0,02

II

112 At5g10300

-0,75

0,06

II

113 At5g03350

-1,32

0,02

II

114 At4g35180

-0,83

0,04

II

115 At4g31870

1,52

0,01

II

116 At4g31500

-0,96

0,02

II

117 At4g27280

-1,61

0,05

II

118 At4g14690

2,95

0,008

II

119 At4g14365

-0,99

0,05

II

120 At4g08850

-0,83

0,06

II

121 At3g57240

-1,39

0,05

II

122 At3g56710

-0,86

0,05

II

123 At3g56400

-0,70

0,06

II

124 At3g55980

-1,02

0,03

II

125 At3g51240

1,95

0,02

II

126 At3g28220

-1,46

0,02

II

127 At3g25780

-1,00

0,05

II

128 At3g22840

1,68

0,00

II

129 At3g16530

-1,49

0,05

II

130 At3g14620

-1,01

0,03

II

131 At3g11820

-0,78

0,05

II

132 At2g47730

-0,65

0,04

II

133 At2g41180

-0,88

0,05

II

134 At2g39210

-0,77

0,05

II

135 At2g38470

-1,42

0,04

II

136 At2g37710

-0,94

0,03

II

137 At2g37040

1,68

0,01

II

138 At2g29350

-0,84

0,03

II

139 At2g28400

-1,076

0,022

II

140 At2g22500

-0,660

0,070

II

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141 At2g18690

-1,38

0,01

II

142 At2g18680

-1,14

0,05

II

143 At2g14610

-1,90

0,04

II

144 At2g14560

-0,868

0,043

II

145 At1g65690

-0,91

0,04

II

146 At1g65560

1,55

0,004

II

147 At1g56060

-1,66

0,02

II

148 At1g51660

-0,636

0,061

II

149 At1g28480

-0,84

0,04

II

150 At1g21250

-1,016

0,022

II

151 At1g19960

-0,81

0,03

-0,647

0,070

II

152 At1g13470

-0,964

0,039

II

153 At1g19180

-0,80

0,05

II

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Supplemental Table S5. Summary of the ANOVA models used to estimate significant effects of the UV treatments, genotype and the interaction UV treatment × genotype.

denDF (denominator degrees of freedom).

A. Gene expression measured by qPCR in Ler and uvr8-2 after 12 h and 36 h outdoors (denDF=10).

CHS 12 h 36 h Source DF F P DF F P UV treatment 2 172.67011 0.0001 2 13.054 0.2684 Genotype 1 2.37137 0.1546 1 194.572 0.0192 UV treatment × Genotype 2 4.37365 0.0432 2 0.508 0.8539 TT7 12 h 36 h Source DF F P DF F P UV treatment 2 444.3359 <.0001 2 1.1729722 0.3486 Genotype 1 0.0396 0.8463 1 0.4131218 0.5348 UV treatment × Genotype 2 16.7865 0.0006 2 0.0321327 0.9685 HY5 12 h 36 h Source DF F P DF F P UV treatment 2 69.74710 <.0001 2 0.869357 0.4486 Genotype 1 59.72127 <.0001 1 1.474632 0.2525 UV treatment × Genotype 2 13.25484 0.0015 2 2.086625 0.1748 COP1 12 h 36 h Source DF F P DF F P UV treatment 2 0.7669407 0.4899 2 0.243269 0.7891 Genotype 1 1.2245187 0.2944 1 4.578354 0.0611 UV treatment × Genotype 2 1.1872110 0.3446 2 0.163553 0.8516 RUP2 12 h 36 h

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Source DF F P DF F P UV treatment 2 101.24126 <.0001 2 8.129416 0.0080 Genotype 1 21.70823 0.0009 1 7.619618 0.0201 UV treatment × Genotype 2 6.96051 0.0128 2 6.013467 0.0193 MEB5.2 12 h 36 h Source DF F P DF F P UV treatment 2 64.14824 <.0001 2 2.445116 0.1366 Genotype 1 21.23567 0.0010 1 6.776489 0.0263 UV treatment × Genotype 2 24.05199 0.0002 2 0.775245 0.4864 UVR8 12 h 36 h Source DF F P DF F P UV treatment 2 2.826950 0.1064 2 0.8664652 0.4497 Genotype 1 1.575138 0.2380 1 1.0666859 0.3260 UV treatment × Genotype 2 1.509540 0.2674 2 0.7068470 0.5163 PDF1.2 12 h 36 h Source DF F P DF F P UV treatment 2 1.572442 0.2548 2 0.443872 0.6536 Genotype 1 26.035006 0.0005 1 5.800154 0.0368 UV treatment × Genotype 2 1.977243 0.1890 2 1.030977 0.3917 PAD3 12 h 36 h Source DF F P DF F P UV treatment 2 14.253639 0.0012 2 3.0009916 0.0953 Genotype 1 9.400364 0.0119 1 1.4275832 0.2597 UV treatment × Genotype 2 0.055472 0.9463 2 0.5473702 0.5949

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RCD1 12 h 36 h Source DF F P DF F P UV treatment 2 2.045437 0.1800 2 0.04520444 0.9560 Genotype 1 4.851114 0.0522 1 0.00027605 0.9871 UV treatment × Genotype 2 1.485548 0.2723 2 0.00510732 0.9949 TAT3 12 h 36 h Source DF F P DF F P UV treatment 2 23.57744 0.0002 2 1.089156 0.3733 Genotype 1 37.26773 0.0001 1 4.181907 0.0681 UV treatment × Genotype 2 0.44426 0.6534 2 0.237446 0.7930 AOXI 12 h 36 h Source DF F P DF F P UV treatment 2 2.0943858 0.1739 2 1.780232 0.2181 Genotype 1 1.3606367 0.2705 1 5.055030 0.0483 UV treatment × Genotype 2 2.4631886 0.1350 2 1.060532 0.3822 VSP1 12 h 36 h Source DF F P DF F P UV treatment 2 3.957300 0.0542 2 0.347251 0.7148 Genotype 1 0.640493 0.4421 1 0.305930 0.5923 UV treatment × Genotype 2 0.482180 0.6311 2 5.075554 0.0301 ATR4 12 h 36 h Source DF F P DF F P UV treatment 2 24.01473 0.0002 2 4.861912 0.0335 Genotype 1 43.75311 0.0001 1 11.577140 0.0067 UV treatment × Genotype 2 0.24188 0.7896 2 4.456384 0.0413

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LOX 12 h 36 h Source DF F P DF F P UV treatment 2 31.165994 0.0001 2 3.150325 0.0869 Genotype 1 19.480321 0.0013 1 3.069358 0.1103 UV treatment × Genotype 2 0.165441 0.8498 2 2.638543 0.1202 AOC3 12 h 36 h Source DF F P DF F P UV treatment 2 36.00602 <.0001 2 3.618573 0.0657 Genotype 1 15.22165 0.003 1 1.638847 0.2294 UV treatment × Genotype 2 1.22991 0.333 2 0.973183 0.4110 NCED3 12 h 36 h Source DF F P DF F P UV treatment 2 3.651143 0.0645 2 0.9178747 0.4305 Genotype 1 0.408353 0.5372 1 0.2800938 0.6082 UV treatment × Genotype 2 0.166253 0.8491 2 0.4680891 0.6393 AOS 12 h 36 h Source DF F P DF F P UV treatment 2 20.857260 0.0003 2 1.862785 0.2053 Genotype 1 13.066562 0.0047 1 3.264195 0.1009 UV treatment × Genotype 2 2.700482 0.10 2 5.159897 0.0289 PMI2 12 h 36 h Source DF F P DF F P UV treatment 2 7.962940 0.0085 2 6.479813 0.0157 Genotype 1 12.462489 0.0054 1 3.553708 0.0888 UV treatment × Genotype 2 2.245307 0.15 2 3.927104 0.0551

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AIF1 12 h 36 h Source DF F P DF F P UV treatment 2 16.75070 0.0006 2 0.6875362 0.5251 Genotype 1 31.71697 0.0002 1 0.0684003 0.7990 UV treatment × Genotype 2 3.22189 0.0832 2 0.6887035 0.5245 SIG5 12 h 36 h Source DF F P DF F P UV treatment 2 101.83965 <.0001 2 2.927703 0.0998 Genotype 1 122.27431 <.0001 1 5.332916 0.0436 UV treatment × Genotype 2 43.89081 <.0001 2 0.965454 0.4136 SPS 12 h 36 h Source DF F P DF F P UV treatment 2 99.66862 <.0001 2 4.662555 0.0371 Genotype 1 52.16762 <.0001 1 10.761346 0.0083 UV treatment × Genotype 2 7.38815 0.0107 2 4.070832 0.0509 CLH1 12 h 36 h Source DF F P DF F P UV treatment 2 2.684271 0.1166 2 1.454934 0.2789 Genotype 1 22.149208 0.0008 1 3.846601 0.0783 UV treatment × Genotype 2 0.032307 0.9683 2 2.710273 0.1147 At5g01520 12 h 36 h Source DF F P DF F P UV treatment 2 251.61130 <.0001 2 5.696182 0.0223 Genotype 1 99.87390 <.0001 1 0.013494 0.9098 UV treatment × Genotype 2 36.84713 <.0001 2 0.602456 0.5662

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DFR 12 h 36 h Source DF F P DF F P UV treatment 2 7.106523 0.0120 2 1.313898 0.3114 Genotype 1 22.274154 0.0008 1 4.309583 0.0646 UV treatment × Genotype 2 16.348878 0.0007 2 3.037992 0.0931 STO 12 h 36 h Source DF F P DF F P UV treatment 2 1.807759 0.2137 2 2.3272456 0.1480 Genotype 1 3.331994 0.0979 1 1.8656667 0.2019 UV treatment × Genotype 2 2.682443 0.1168 2 0.1905582 0.8294 GRX480 12 h 36 h Source DF F P DF F P UV treatment 2 6.45797 0.0158 2 2.0846935 0.1751 Genotype 1 32.45098 0.0002 1 0.0073179 0.9335 UV treatment × Genotype 2 0.22308 0.8039 2 0.7751476 0.4864

B. Gene expression measured by qPCR in Ler and uvr8-2 after three weeks (denDF=8). CHS Source DF F P UV treatment 2 0.705172 0.5223 Genotype 1 7.048953 0.0290 UV treatment × Genotype 2 0.165677 0.8502 HAT2 Source DF F P

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UV treatment 2 19.19738 0.0009 Genotype 1 58.57053 0.0001 UV treatment × Genotype 2 3.09454 0.1011 AOC3 Source DF F P UV treatment 2 1.0097451 0.4064 Genotype 1 0.0730799 0.7937 UV treatment × Genotype 2 0.0612972 0.9410 RUP2 Source DF F P UV treatment 2 72.54591 <.0001 Genotype 1 161.54599 <.0001 UV treatment × Genotype 2 0.55976 0.5922 ATR4 Source DF F P UV treatment 2 0.4836175 0.6335 Genotype 1 0.0402518 0.8460 UV treatment × Genotype 2 1.6020343 0.2599 HY5 Source DF F P UV treatment 2 0.6946817 0.5270 Genotype 1 0.9297521 0.3632 UV treatment × Genotype 2 2.6642248 0.1298 LOX Source DF F P UV treatment 2 2.307673 0.1617

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Genotype 1 1.425468 0.2667 UV treatment × Genotype 2 0.012216 0.9879 TAT3 Source DF F P UV treatment 2 0.9103042 0.4404 Genotype 1 0.9489398 0.3585 UV treatment × Genotype 2 0.3503167 0.7148 WRKY70 Source DF F P UV treatment 2 1.112379 0.3748 Genotype 1 4.738432 0.0512 UV treatment × Genotype 2 2.569531 0.1374 AOS Source DF F P UV treatment 2 1.4495494 0.2903 Genotype 1 0.4679975 0.5132 UV treatment × Genotype 2 0.0222020 0.9781 JAZ1 Source DF F P UV treatment 2 1.053498 0.3925 Genotype 1 10.255788 0.0126 UV treatment × Genotype 2 0.227208 0.8017 PR1 Source DF F P UV treatment 2 0.1834873 0.8358 Genotype 1 1.3563665 0.2777

(32)

UV treatment × Genotype 2 1.5530014 0.2692 DFR Source DF F P UV treatment 2 0.071083 0.9320 Genotype 1 3.839792 0.0857 UV treatment × Genotype 2 0.634627 0.5549 GRX480 Source DF F P UV treatment 2 1.738338 0.2361 Genotype 1 12.543766 0.0076 UV treatment × Genotype 2 1.758114 0.2329 PMI2 Source DF F P UV treatment 2 0.453038 0.6510 Genotype 1 5.871699 0.0416 UV treatment × Genotype 2 1.144335 0.3655

C. Accumulation of PDX1 measured by western blot in Ler and uvr8-2 after 12 h and 36 h (denDF=25).

12 h 36 h

Source DF F P DF F P

UV treatment 2 20.876 <.0001 2 33.076 <.0001

Genotype 1 0.015 0.90 1 33.230 <.0001

UV treatment × Genotype 2 0.468 0.63 2 8.529 0.0015

(33)

Phe 12 h 36 h Source DF F P DF F P UV treatment 2 3.8400 0.0398 2 1.71566 0.2053 Genotype 1 6.6619 0.0183 1 9.20459 0.0066 UV treatment × Genotype 2 2.3483 0.1226 2 5.11335 0.0161 Q-3-N-7-Rha 12 h 36 h Source DF F P DF F P UV treatment 2 18.95817 <.0001 2 45.59767 <.0001 Genotype 1 12.05807 0.0025 1 0.00116 0.9731 UV treatment × Genotype 2 7.80696 0.0034 2 1.32161 0.2890 K-3-O-S-7-O-Glu 12 h 36 h Source DF F P DF F P UV treatment 2 27.14600 <.0001 2 30.82153 <.0001 Genotype 1 44.83310 <.0001 1 0.14295 0.7094 UV treatment × Genotype 2 14.25677 <.0002 2 3.05743 0.0694 K-NeoHes-3-R-7 12 h 36 h Source DF F P DF F P UV treatment 2 1.4083 0.2690 2 2.71580 0.0905 Genotype 1 3.9648 0.0610 1 2.22179 0.1517 UV treatment × Genotype 2 3.0298 0.0721 2 2.19730 0.1372 Chlorogenic acid 12 h 36 h Source DF F P DF F P UV treatment 2 38.09202 <.0001 2 6.25680 0.0078 Genotype 1 15.63075 0.0009 1 1.98548 0.1742 UV treatment × Genotype 2 9.85546 0.0012 2 2.08134 0.1510

(34)

Q-3-R-7-Rha 12 h 36 h Source DF F P DF F P UV treatment 2 36.78064 <.0001 2 43.46911 <.0001 Genotype 1 3.79848 0.0662 1 2.99556 0.0989 UV treatment × Genotype 2 3.50829 0.0505 2 1.83688 0.1852 K-3-S-Rha-7 12 h 36 h Source DF F P DF F P UV treatment 2 0.34514 0.7125 2 2.96065 0.0748 Genotype 1 0.32094 0.5777 1 0.02211 0.8833 UV treatment × Genotype 2 2.35317 0.1222 2 1.92171 0.1724 K-3-R 12 h 36 h Source DF F P DF F P UV treatment 2 19.1814 <.0001 2 7.36691 0.0040 Genotype 1 4.3731 0.0502 1 0.81953 0.3761 UV treatment × Genotype 2 2.9045 0.0793 2 1.84014 0.1847 Q-3-(O)-Rha 12 h 36 h Source DF F P DF F P UV treatment 2 6.07237 0.0091 2 27.14106 <.0001 Genotype 1 0.18800 0.6695 1 0.58472 0.4534 UV treatment × Genotype 2 4.27470 0.0293 2 1.53053 0.2407 IsoRha-3-O-G-7-O-Rha 12 h 36 h Source DF F P DF F P UV treatment 2 1.08687 0.3584 2 14.11396 0.0002 Genotype 1 10.76918 0.0041 1 11.77962 0.0026 UV treatment × Genotype 2 0.02176 0.9785 2 0.79247 0.4664 Q-3-Glu 12 h 36 h

(35)

Source DF F P DF F P UV treatment 2 42.44323 <.0001 2 152.4937 <.0001 Genotype 1 0.68743 0.4179 1 0.0093 0.9243 UV treatment × Genotype 2 0.02636 0.9740 2 18.4070 <.0001 K-R-3-Rha-7 12 h 36 h Source DF F P DF F P UV treatment 2 1.63453 0.2213 2 7.5955 0.0035 Genotype 1 0.11850 0.7344 1 0.2442 0.6266 UV treatment × Genotype 2 0.55383 0.5837 2 4.2906 0.0282 K-Rha-3-Rha-7 12 h 36 h Source DF F P DF F P UV treatment 2 6.6916 0.0063 2 1.48005 0.2515 Genotype 1 13.3209 0.0017 1 3.28729 0.0849 UV treatment × Genotype 2 2.79502 0.0863 2 1.21910 0.3165 K-3-O-Glu 12 h 36 h Source DF F P DF F P UV treatment 2 18.9103 <.0001 2 7.83289 0.0031 Genotype 1 10.1016 0.0049 1 0.42455 0.5221 UV treatment × Genotype 2 1.8242 0.1885 2 5.09233 0.0163 Quercetin 12 h 36 h Source DF F P DF F P UV treatment 2 1.89637 0.1824 2 18.3129 <.0001 Genotype 1 1.75363 0.2040 1 3.9363 0.0611 UV treatment × Genotype 2 3.11649 0.0719 2 11.1683 0.0006 Kaempferol 12 h 36 h Source DF F P DF F P

(36)

UV treatment 2 1.2688 0.3051 2 2.6233 0.0973 Genotype 1 2.8760 0.1071 1 0.8762 0.3604 UV treatment × Genotype 2 1.1734 0.3319 2 3.5457 0.0481 Unidentified 12 h 36 h Source DF F P DF F P UV treatment 2 75.90047 <.0001 2 75.90047 <.0001 Genotype 1 89.48351 <.0001 1 89.48351 <.0001 UV treatment × Genotype 2 95.07576 <.0001 2 95.07576 <.0001 Tyr 12 h 36 h Source DF F P DF F P UV treatment 2 14.00537 0.0002 2 3.53442 0.0485 Genotype 1 6.83222 0.0171 1 9.49409 0.0059 UV treatment × Genotype 2 4.80939 0.0204 2 3.12159 0.0553 Trp 12 h 36 h Source DF F P DF F P UV treatment 2 0.51007 0.6084 2 6.02226 0.0090 Genotype 1 0.40881 0.5302 1 7.01633 0.0154 UV treatment × Genotype 2 1.47889 0.2530 2 6.23188 0.0079 Kynurenic acid 12 h 36 h Source DF F P DF F P UV treatment 2 5.77605 0.0110 2 2.239983 0.1325 Genotype 1 2.56312 0.1259 1 0.347514 0.5621 UV treatment × Genotype 2 3.43436 0.0533 2 0.175288 0.8405

(37)

Source DF F P DF F P UV treatment 2 4.12653 0.0335 2 12.18607 0.0003 Genotype 1 1.02588 0.3246 1 2.25228 0.1490 UV treatment × Genotype 2 2.27314 0.1318 2 1.28494 0.2985 p -Coumaric acid 12 h 36 h Source DF F P DF F P UV treatment 2 1.96780 0.1687 2 1.71208 0.2059 Genotype 1 5.84046 0.0265 1 2.45639 0.1327 UV treatment × Genotype 2 2.43129 0.1162 2 1.73925 0.2012 Sinapic acid 12 h 36 h Source DF F P DF F P UV treatment 2 0.89987 0.4233 2 4.4136 0.0258 Genotype 1 8.75732 0.0081 1 4.3017 0.0512 UV treatment × Genotype 2 2.52783 0.1063 2 0.5876 0.5650

Ferulic acid hexose 12 h 36 h

Source DF F P DF F P UV treatment 2 3.99542 0.0356 2 9.3332 0.0014 Genotype 1 5.12293 0.0355 1 2.6787 0.1173 UV treatment × Genotype 2 1.23566 0.3130 2 0.7576 0.4818 Camalexin 12 h 36 h Source DF F P DF F P UV treatment 2 14.98064 0.0001 2 7.258467 0.0043 Genotype 1 5.32285 0.0325 1 1.915437 0.1816 UV treatment × Genotype 2 2.18768 0.1396 2 0.289755 0.7515

(38)

E. Optical measurements in Ler and uvr8-2 after 50 h (denDF=13). Chlorophyll (SPAD) Source DF F P UV treatment 2 1.246 0.3198 Genotype 1 21.312 0.0005 UV treatment × Genotype 2 0.949 0.4123 HCA (Dualex 315nm) Source DF F P UV treatment 2 159.544 <.0001 Genotype 1 36.246 <.0001 UV treatment × Genotype 2 5.311 0.0206 Flavonoids (Dualex 375nm) Source DF F P UV treatment 2 91.3896 <.0001 Genotype 1 68.5581 <.0001 UV treatment × Genotype 2 10.6819 0.0018

E. Number of flowering plants counted after three weeks (denDF=16).

Source DF F P

UV treatment 2 4.29182 0.0322

Genotype 1 16.80043 <.001

(39)

Supplemental Table S6. Tests of significance of effects of the UV treatments on gene expression, accumulation of PDX1 and metabolites, and optical measurements.

A1, A2, A3, and A4. P values for the comparisons between the UV treatments.

Contrasts amongst individual treatments were fitted within genotypes when the interaction UV Treatment x Genotype was significant.

When ANOVA only showed significant main effects of the UV treatments and genotypes but not a significant interaction contrasts between the UV treatments were fitted pooling the genotypes .

DF=degrees of freedom.

A1. Genes showing significant interaction UV treatment × genotype in Ler and uvr8-2 after 12 h (DF=10) Wild-type UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B

HY5 < 0.001 0.085 0.002 MEB5.2 0.019 0.199 0.307 SPS < 0.001 0.002 0.036 RUP2 < 0.001 < 0.001 0.130 At5g01520 < 0.001 < 0.001 0.002 SIG5 < 0.001 0.256 < 0.001 CHS < 0.001 < 0.001 0.210 TT7 < 0.001 < 0.001 0.313 DFR < 0.001 < 0.001 0.34

uvr8-2 UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B

HY5 < 0.001 < 0.001 0.781 MEB5.2 < 0.001 < 0.001 0.329 SPS < 0.001 < 0.001 0.647 RUP2 < 0.001 < 0.001 0.035 At5g01520 < 0.001 < 0.001 0.173 SIG5 < 0.001 < 0.001 0.097 CHS < 0.001 < 0.001 0.849

(40)

TT7 < 0.001 < 0.001 0.313

DFR 0.99 0.36 0.36

A2. Genes where ANOVA only showed significant main effects of the UV treatments and genotypes in Ler and uvr8-2 after 12 h (DF=10)

UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B VSP1 0.515 0.125 0.040 AOS 0.720 0.0055 0.0030 RCD1 0.119 0.421 0.407 CLH1 0.622 0.284 0.132 ATR4 0.003 < 0.001 0.359 PDF1.2 0.213 0.305 0.036 AOC3 0.001 < 0.001 0.337 TAT3 0.008 0.002 0.465 LOX 0.001 < 0.001 0.453 PAD3 0.048 0.004 0.184 PMI2 0.05 0.41 0.22 AIF1 0.003 0.48 0.01 GRX480 0.07 0.06 0.95

A3. Genes showing significant interaction UV treatment × genotype in Ler and uvr8-2 after 36 h (DF=10) Wild-type UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B

VSP1 ns ns ns SPS 0.695 0.622 0.167 RUP2 1.00 1.00 1.00 PMI2 0.809 0.809 0.797 AOS 0.999 0.999 0.999 ATR4 1.00 1.00 1.00

(41)

uvr8-2 UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B VSP1 ns ns ns SPS 0.050 0.115 0.695 RUP2 0.005 0.005 1.00 PMI2 0.010 0.065 0.796 AOS 0.040 0.194

0.998

ATR4 0.015 0.035

1.000

A4. Genes where ANOVA only showed significant main effects of the UV treatments and genotypes in Ler and uvr8-2 after 36 h (DF=10)

UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B AOXI 0.410 0.972 0.392 SIG5 0.550 0.716 0.344 At5g01520 0.088 0.258 0.506 MEB5.2 0.528 0.226 0.080 CHS 0.552 0.180 0.429 PDF1.2 0.522

0.859

0.640

A5. Significance of simple effects of the genotype in models fit separately to each UV treatment for genes showing significant interaction UV treatment x Genotype after 12 and 36 h outdoors (DF=2)

12 h

gen. wihtin UV 0

gen. within UV A

gen. within UV A+B

HY5 0.2954

0.0745

0.0176

MEB5.2 0.1201

0.0115

0.0223

SPS 0.1542

0.0248

0.0481

RUP2 0.1753

0.2543

0.0098

At5g01520 0.868

0.0250

0.0013

SIG5 0.8132

0.0281

0.0021

CHS 0.3998

0.2075

0.0944

TT7 0.0587

0.2032

0.0479

(42)

DFR 0.4543

0.1163

0.0038

36h

gen. whithin UV 0

gen. within UV A

gen. within UV A+B

VSP1 0.0453

0.2124

0.9425

SPS 0.8332

0.0168

0.0211

RUP2 0.0775

0.0459

0.1143

PMI2 0.3566

0.1296

0.0465

AOS 0.1573

0.0490

0.2502

ATR4 0.5624

0.0358

0.0289

B. PDX1 accumulation in Ler and uvr8-2 after 12 and 36 h outdoors (DF=25) 12 h UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B

PDX1 < 0.001 < 0.001 0.64

36 h

Wild-type UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B

PDX1 0.01 0.004 0.57

PDX1 < 0.001 < 0.001 0.01

C. Metabolites detected by UPLC-MS/MS in Ler and uvr8-2

C1. Metabolites showing significant interaction UV treatment × genotype after 12 h (DF=19) Wild-type UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B

Q-3-N-7-Rha < 0.001 < 0.001 0.18

K-3-O-S-7-O-Glu < 0.001 < 0.001 0.10

Chlorogenic acid 0.33 0.33 0.01

(43)

Q-3-(O)-Rha 0.05 < 0.001 0.44

unidentified 0.99 < 0.001 < 0.001

Tyr 1.0 1.0 0.64

kynurenic acid 0.68 0.12 0.22

Q-3-N-7-Rha 0.78 0.29 0.78 K-3-O-S-7-O-Glu 0.95 0.95 0.95 Chlorogenic acid 0.05 0.33 0.33 Q-3-R-7-Rha 0.07 0.01 0.25 Q-3-(O)-Rha 1.0

1.0

unidentified 1.0

1.0

Tyr 0.49

1.0

kynurenic acid 0.03 0.3 0.22

C2. Metabolites where ANOVA only showed significant main effect of the UV treatments and genotypes after 12 h (DF=19)

UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B Phe 0.21 0.84 0.13 K-3-R 0.07 0.23 0.48 IsoRha-3-O-G-7-O-Rha 0.40 0.27 0.80 Q-3-Glu < 0.001 < 0.001 0.68 K-Rha-3-Rha-7 0.34 0.44 0.08 K-3-O-Glu 0.008 0.03 0.49

12-hydroxy jasmonic acid 12-hexose 0.005 0.49 0.01

(44)

12-hydroxy jasmonic acid 12-hexose

Treatment Genotype Mean se

UV 0 Ler 0.008 0,004 UV A Ler 0.019 0,01 UV A+B Ler 0.011 0,005 UV 0 uvr8 0.013 0,01 UV A uvr8 0.016 0,01 UV A+B uvr8 0.016 0,01

C3. Metabolites showing significant interaction UV treatment × genotype after 36 h (DF=19) Wild-type UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B

Phe 0.72 0.72 0.83 Q-3-Glu < 0.001 < 0.001 < 0.001 K-R-3-Rha-7 1.0 1.0 1.0 K-3-O-Glu 0.31 0.01 0.22 Quercetin < 0.001 < 0.001 < 0.001 Kaempferol 1.0 1.0 1.0 unidentified 1.0 < 0.001 < 0.001 Tyr 0.85 0.38 0.97 Trp 1.0 1.0 1.0

Phe 0.01 0.72 0.19 Q-3-Glu < 0.001 < 0.001 < 0.001 K-R-3-Rha-7 0.001 1.0 0.008 K-3-O-Glu 0.008 0.31 0.01 Quercetin < 0.001 < 0.001 < 0.001 Kaempferol 0.05 1.0 0.03 unidentified 1.0 1.0 1.0 Tyr 0.14 0.97 0.04

(45)

Trp 0.001 1.0 0.003

C4. Metabolites where ANOVA only indicated significant main effect of the UV treatments and genotypes after 36 h (DF=19)

UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B Q-3-N-7-Rha < 0.001 < 0.001 0.64 K-3-O-S-7-O-Glu < 0.001 < 0.001 0.66 Chlorogenic acid 0.57 0.03 0.05 Q-3-R-7-Rha < 0.001 < 0.001 0.33 K-3-R 0.07 0.03 0.71 Q-3-(O)-Rha < 0.001 < 0.001 0.79 IsoRha-3-O-G-7-O-Rha 0.01 0.01 0.39

Ferulic acid hexose 0.23 0.01 0.17

12-hydroxy jasmonic acid 12-hexose 0.01 0.09 0.44

12-hydroxy jasmonic acid 12-hexose

UV 0 Ler 0.011 0,005 UV A Ler 0.021 0,01 UV A+B Ler 0.018 0,008 UV 0 uvr8 0.014 0,01 UV A uvr8 0.032 0,01 UV A+B uvr8 0.018 0,01 IsoRha-3-O-G-7-O-Rha

UV 0 Ler 0,039 0,017

UV A Ler 0,110 0,049

UV A+B Ler 0,146 0,066

(46)

UV A uvr8 0,275 0,124

UV A+B uvr8 0,182 0,082

D. Optical measurements in Ler and uvr8-2 after 50 h outdoors (DF=13)

Wild-type UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B

Dualex 375nm < 0.001 < 0.001 < 0.001

Dualex 315nm 0.65 0.005 0.04

Dualex 375nm < 0.001 < 0.001 0.87 Dualex 315nm 0.65 0.75 0.75 UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B SPAD 0.64

0.63

0.48

SPAD averages Wild-type uvr8-2 UV A+B 26.27 22.97 UV A 27.35 24.102 UV 0 27.033 22.57

E. Genes where ANOVA only showed significant main effects of the UV treatments and genotypes in Ler and uvr8-2 after three weeks outdoors (DF=8)

UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B

HAT2 0.068 0.052 0.851

CHS 0.722 0.765 0.471

(47)

WRKY70 0.85 0.71 0.87

JAZ1 0.81 0.94 0.73

PMI2 0.93 0.29 0.21

At1g28480 0.07 0.89 0.04

F. Number of flowering plants per pot counted after three weeks (DF=16)

UV 0 vs UV A UV 0 vs UV A+B UV A vs UV A+B

(48)

Supplemental Table S7. Metabolites identified in wild-type and uvr8-2 leaves by UPLC-MS. Retention times (RT) and detected masses (M+1) are shown.