Novel Technologies for Recombinant Protein Overexpression in Escherichia coli
Tobias Cornvik
Department of Biochemistry and Biophysics Stockholm University
2006=
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Abstract
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Table of contents
^_pqo^`q KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK P q^_ib=lc=`lkqbkqp KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK Q ifpq=lc=m^mbop KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK R
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List of papers
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Table 1 The genetic code. Nucleotides are shown as their single letter code and the amino acid each triplet codes for is shown to the right. The genetic code is conserved between different species. A gene coding for a human protein can therefore be transferred to a bacterium and still be correctly translated.
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éêçíÉáå=~êÉ=ìëì~ääó=ÇáîáÇÉÇ=áåíç=Ñçìê=Å~íÉÖçêáÉëK=qÜÉ=éêáã~êó=ëíêìÅíìêÉ=
áë=íÜÉ=ëÉèìÉåÅÉ=çÑ=~ãáåç=~ÅáÇë=áå=íÜÉ=éçäó=éÉéíáÇÉ=ÅÜ~áåI=ÅçååÉÅíÉÇ=íç=
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íç= äçÅ~ä= ÅçåÑçêã~íáçåë= çÑ= íÜÉ= éçäó= éÉéíáÇÉ= ÅÜ~áåK= qÜÉ= íïç= ãçëí=
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ÅçåëáëíáåÖ= çÑ= ÜóÇêçÖÉå= ÄçåÇëK= eçï= íÜÉëÉ= ëÉÅçåÇ~êó= ëíêìÅíìê~ä=
ÉäÉãÉåíë=~êÉ=~êê~åÖÉÇ=áå=íÜêÉÉ=ÇáãÉåëáçå~ä=ëé~ÅÉ=áë=íÉêãÉÇ=íÜÉ=íÉêíá~êó=
ëíêìÅíìêÉ=çê=ÑçäÇK=qÜÉ=ä~ëí=äÉîÉä=çÑ=ëíêìÅíìê~ä=~êê~åÖÉãÉåí=áå=~=éêçíÉáå=
áë= íÜÉ= èì~íÉêå~êó= ëíêìÅíìêÉ= ïÜáÅÜ= ÇÉëÅêáÄÉë= Üçï= ëÉîÉê~ä= éçäóéÉéíáÇÉ=
ÅÜ~áåë=Å~å=áåíÉê~Åí=~åÇ=Ñçêã=~=Ñáå~ä=ãìäíáJëìÄìåáí=éêçíÉáåK==
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^åçíÜÉê= íóéÉ= çÑ= ëíêìÅíìê~ä= ÉäÉãÉåí= ïáíÜáå= ~= éêçíÉáå= ~êÉ= Ççã~áåëK= ^=
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~åÇ= áë= ëÉäÑ= ëí~ÄáäáòáåÖK= píêìÅíìê~ä= Ççã~áåë= Åçåëáëí= çå= ~îÉê~ÖÉ= çÑ= NMM=
~ãáåç= ~ÅáÇë= ~åÇ= ~êÉ= îÉêó= ëÉäÇçã= ä~êÖÉê= íÜ~å= PMM= ~ãáåç= ~ÅáÇë= xQzK=
pÉîÉê~ä=ëíêìÅíìê~ä=Ççã~áåë=~êÉ=ìëì~ääó=ÅçãÄáåÉÇ=Äó=ÑäÉñáÄäÉ=äáåâÉêë=íç=
Ñçêã= íÜÉ= Ñáå~ä= íÜêÉÉ= ÇáãÉåëáçå~ä= ëíêìÅíìêÉ= çÑ= íÜÉ= éêçíÉáåK= ^= éêçíÉáå=
Ççã~áå= áë= íóéáÅ~ääó= ~ëëçÅá~íÉÇ= ïáíÜ= ~= ëéÉÅáÑáÅ= ÑìåÅíáçå= ÉKÖK= ~å=
Éåòóã~íáÅ=~Åíáîáíó=çê=ëéÉÅáÑáÅ=áåíÉê~Åíáçå=éêçéÉêíáÉëK==
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A Historical Perspective
Cloning and Protein Production
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Structural Biology
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j~ñ= îçå= i~ìÉ= EkçÄÉä= éêáòÉ= ä~ìêÉ~íÉ= NVNQF= ÇáëÅçîÉêÉÇ= íÜ~í= Åêóëí~äë=
ÇáÑÑê~ÅíÉÇ= uJê~óëK= c~íÜÉê= ~åÇ= ëçåI= páê= táääá~ã= eÉåêó= _ê~ÖÖ= ~åÇ=
táääá~ã= i~ïêÉåÅÉ= _ê~ÖÖI= éáçåÉÉêÉÇ= ÉÑÑçêíë= áå= íÜÉ= ~å~äóëáë= çÑ= Åêóëí~ä=
ëíêìÅíìêÉë=Äó=uJê~ó=ÇáÑÑê~ÅíáçåI=~åÇ=ïÉêÉ=~ï~êÇÉÇ=íÜÉ=kçÄÉä=mêáòÉ=áå=
mÜóëáÅë=áå=NVNRK=
få=NVPT=j~ñ=mÉêìíò=ã~ÇÉ=íÜÉ=Ñáêëí=ÉñéÉêáãÉåíë=íç=áåîÉëíáÖ~íÉ=áÑ=áí=ï~ë=
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ï~ë= íÜÉ= ìëÉ= çÑ= ÅêóçJÅççäáåÖ= ~åÇ= Ñä~ëÜ= ÑêÉÉòáåÖ= çÑ= Åêóëí~äë= áå= íÜÉ=
ÄÉÖáååáåÖ= çÑ= íÜÉ= NVVMëK= qÜÉ= áåíêçÇìÅíáçå= çÑ= åÉï= éÜ~ëáåÖ= ãÉíÜçÇë=
ëìÅÜ= ~ë= ãìäíáï~îÉäÉåÖíÜ= ~åçã~äçìë= ÇáÑÑê~Åíáçå= Ej^aF= çê= ëáåÖäÉ=
ï~îÉäÉåÖíÜ=~åçã~äçìë=ÇáÑÑê~Åíáçå=Ep^aF=Ü~îÉ=~äëç=ÅçåíêáÄìíÉÇ=ÖêÉ~íäó=
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0 10000 20000 30000 40000
1973 1975
1977 1979
1981 1983
1985 1987
1989 1991
1993 1995
1997 1999
2001 2003
2005 Year
Number of Structures
Deposited structures for the year Total available structures
=
Figure 1 Number of structures deposited in the Protein Data Bank. Data obtained from the PDB website.
=
Membrane Proteins
^ää=äáîáåÖ=ÅÉääë=~êÉ=ëìêêçìåÇÉÇ=Äó=~=íÜáå=ãÉãÄê~åÉ=ÅçãéçëÉÇ=çÑ=äáéáÇëK=
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EcáÖìêÉ= OFK= få= NVTO= páåÖÉê= ~åÇ= káÅÜçäëçå= éêçéçëÉÇ= íÜÉ= ÑäìáÇ= ãçë~áÅ=
ãçÇÉäI=ïÜáÅÜ=ïáíÜ=ëçãÉ=ãçÇáÑáÅ~íáçåëI=áë=ëíáää=íçÇ~ó=íÜÉ=ãçÇÉä=ìëÉÇ=íç=
ÇÉëÅêáÄÉ= ÄáçäçÖáÅ~ä= ãÉãÄê~åÉë= xOSzK= qÜáë= ãçÇÉä= êÉí~áåë= íÜÉ= äáéáÇ=
Äáä~óÉêI= Äìí= íÜÉ= éêçíÉáåJäáéáÇJéêçíÉáå= ë~åÇïáÅÜ= ï~ë= êÉéä~ÅÉÇ= Äó= ~=
ãçÇÉä=ïÜÉêÉ=íÜÉ=éêçíÉáåë=“Ñäç~íÒ=áå=íÜÉ=ãÉãÄê~åÉ=EcáÖìêÉ=OFK==
=
Danielli and Davson 1935 Singer and Nicolson 1972
Protein Integral protein
Peripheral protein
a b
=
Figure 2 Schematic outline of the different lipid bilayer models. (A) The model suggested by Danelli and Davson. In this model the lipid is organised in a bilayer surrounded by a protein layer. (B) The fluid mosaic model suggested by Singa and Nicolson. The lipids are arranged in bilayer but the proteins can span the bilayer.
=
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Structural Genomics
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