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Molecular Biotechnology Programme

Uppsala University School of Engineering

UPTEC X 05 043 Date of issue 2005-08

Author

Erik Holmqvist

Title (English)

Functional analysis of chromosomally encoded small regulatory RNAs in E. coli

Title (Swedish)

Abstract

The E. coli small regulatory RNA MicA has here been shown to act as an antisense RNA targeting ompA-mRNA in vivo. Reporter gene fusions further showed that over-expression of the sRNAs MicA and MicF leads to decreased translation of lrp-mRNA, encoding the leucin responsive regulator protein.

Keywords

sRNA, post-transcriptional regulation, reporter gene system, structural probing Supervisors

Gerhart Wagner

Department of Cell and Molecular Biology, Uppsala University

Scientific reviewer

Fredrik Söderbom

Departement of Molecular Biology, Swedish University of Agricultural Sciences

Project name Sponsors

Language

English

Security

ISSN 1401-2138

Classification

Supplementary bibliographical information Pages

23

Biology Education Centre Biomedical Center

Husargatan 3 Uppsala Box 592 S-75124 Uppsala Tel +46 (0)18 4710000 Fax +46 (0)18 555217

References

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