Structural and Functional Studies of Membrane Proteins

The aim of this project was to express N- and C-terminal labeled CAH1 proteins in Arabidopsis cells, analyse their expression level and localisation and finally, to purify

The relatively stable constructs were setup for crystallization with commercial screens, with different additives, such as substrates of this enzme, NADH, or some

Lanes 7-11 are the eluted fractions, the target protein is not purified. The right SDS picture explains the refolding process of M2-3 inclusion bodies using dialysis.. Lane 1

Along with these two proteins other enzymes have been identified, such as ribonucleotide reductase, which is responsible for generating DNA, and Cysteinyl-tRNA synthetase, which

Clones were analyzed with analytical PCR (Figure 7). Positive clones were identified as expressing single strong bands of the desired length of 1005 bp. Then were then tested

It functions by blocking the release of elongation factor G (EF-G) from the ribosome, thus preventing the binding of a new aminoacyl tRNA to the ribosome and blocking

To test if FusB could bind to S.a EF-G, purified FusB and S.a EF-G were mixed at different molar ratios in FusB gel filtration buffer (20mM Tris-HCl, 300mM NaCl,

We ®nd that N-terminally ¯anking resi- dues have no effect on helical hairpin formation in our model protein (possibly because the lumenal.. or cytoplasmic location of the N terminus