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Molecular Biotechnology Programme Uppsala University School of Engineering

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Molecular Biotechnology Programme

Uppsala University School of Engineering

UPTEC X 12 009 Date of issue 2012-06

Author

Caroline Larsson

Title (English)

Bacterial Sortase A as a drug target

Title (Swedish)

Abstract

Sortase A is a housekeeping enzyme of Gram-positive bacteria that catalyses the anchoring of surface proteins to the bacterial peptidoglycan. The enzyme works to establish an interaction between bacteria and host cells and is essential for pathogenesis. This makes Sortase A a potential suitable target for inhibition, in order to treat bacterial infections.

In this degree project Sortase A from Staphylococcus aureus was explored and potential inhibitors were investigated by performing enzyme activity and bacterial binding assays. A robust FRET assay was developed and optimized for a recombinant version of the enzyme and serves as a good starting point for studying inhibition.

Keywords

Antibiotic resistance, Sortase A, Staphylococcus aureus, Gram-positive bacteria, inhibition, FRET assay, fibronectin-binding

Supervisors

Ian Henderson

Medivir AB

Scientific reviewer

Helena Danielsson

Uppsala University

Project name Sponsors

Language

English

Security

ISSN 1401-2138

Classification

Supplementary bibliographical information Pages

49

Biology Education Centre Biomedical Center

Husargatan 3 Uppsala Box 592 S-75124 Uppsala Tel +46 (0)18 4710000 Fax +46 (0)18 471 4687

References

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