• No results found

Molecular Biotechnology Programme Uppsala University School of Engineering

N/A
N/A
Protected

Academic year: 2022

Share "Molecular Biotechnology Programme Uppsala University School of Engineering"

Copied!
1
0
0

Loading.... (view fulltext now)

Full text

(1)

Molecular Biotechnology Programme

Uppsala University School of Engineering

UPTEC X 07 003 Date of issue 2007-01 Author

Martin Holmgren

Title (English)

Biomolecular interaction studies of xanthurenic acid using capillary electrophoresis

Abstract

Xanthurenic acid, which is a metabolite product emanating from the degradation of

tryptophan by the enzyme indoleamine-2,3 dioxygenase (IDO), is present in our body and can induce cell death and also denaturation of some proteins by binding to them. The primary goal of this project was to study the interaction between xanthurenic acid and insulin at

physiologic pH. The affinity of xanthurenic acid to calcitonin and cyclodextrins was also determined. To study the mentioned associations, capillary electrophoresis was used where focus was on the partial filling technique. The xanthurenic acid and insulin interacted weakly whereas no interaction was detected between the acid and calcitonin. The affinity of

xanthurenic acid to γ-cyclodextrin and 2-hydroxypropyl β-cyclodextrin was determined to 60 M-1 and 90 M-1, respectively. Furthermore, it was shown that the 2-hydroxypropyl groups facilitated the binding of xanthurenic acid to 2-hydroxypropyl β-cyclodextrin.

Keywords

Capillary electrophoresis, partial filling technique, association constants, xanthurenic acid, insulin, calcitonin, cyclodextrin

Supervisors

Ahmad Amini Medical Products Agency Scientific reviewer

Douglas Westerlund

Division of Analytical Pharmaceutical Chemistry, Uppsala University

Project name Sponsors

Language

English

Security

ISSN 1401-2138 Classification

Supplementary bibliographical information Pages

28

Biology Education Centre Biomedical Center Husargatan 3 Uppsala Box 592 S-75124 Uppsala Tel +46 (0)18 4710000 Fax +46 (0)18 555217

References

Related documents

It showed large dynamic range, no measureable non-specific binding and high sensitivity (with linear range around 0.1 – 10 µg/ml depending on the proteins). The method

Escherichia coli, 23S rRNA, Post-transcriptional modification, In vitro, Peptidyl transferase centre, domain V, Methylation, Pseudouridine, Reverse

The fibrinolytic enzyme tissue type plasminogen activator (t-PA) is released by the vascular endothelium to limit the growth of blood clots and ultimately prevent the occurrence of

investigate the presence of P4 in the brain, in neuromuscular junctions and in adrenal glands, the colocation between P4 and VAChT respectively P4 and VMAT and the possibility that P4

The information obtained by using these algorithms could contribute to the development of new drugs by generating new ligands that target specific high-energy , unfavorable

Results from the project shows that when multiplexing there are differences in measured IgE- levels between an existing singleplex method and the multiplex prototype. The project also

A method for measuring cell concentration and identity based on flow cytometry (FCM) and fluorescent marking is developed and subsequently compared with traditional plating based

Here we have attempted to produce the serine proteases rat mast cell protease 2 and mouse mast cell protease 5 in a culture of HEK 293 cells; and mouse mast cell protease 4,