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Molecular Biotechnology Programme Uppsala University School of Engineering

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Molecular Biotechnology Programme

Uppsala University School of Engineering

UPTEC X 08 022 Date of issue 2008-05 Author

Gabriella Edfeldt

Title (English)

Development and evaluation of new reagents for in situ Proximity Ligation Assay (PLA)

Title (Swedish)

Abstract

In situ Proximity Ligation Assay (PLA) is a novel method for detection of proteins, protein interactions and protein modifications. The aim of this study was to investigate the use of streptavidin as generalized secondary reagent for in situ PLA. The work also includes evaluation of direct conjugated primary antibodies as PLA probes. Both assays show promising results but need further optimization.

Keywords

ErbB receptor family, Immunostaining, Proximity Ligation Assay, Streptavidin/Biotin Supervisors

Dr. Mats Gullberg Olink Bioscience

Scientific reviewer

Dr. Ola Söderberg

Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University

Project name Sponsors

Language

English

Security

ISSN 1401-2138 Classification

Supplementary bibliographical information Pages

20

Biology Education Centre Biomedical Center Husargatan 3 Uppsala Box 592 S-75124 Uppsala Tel +46 (0)18 4710000 Fax +46 (0)18 555217

References

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