1
Development of Enhanced Molecular Diagnostics tools for Protein Detection
and Analysis
TONGE EBAI
2
Figure 4.3.1: Affinity-based immunoassays formats development timeline and some properties
The table below demonstrates (a) effects of cross reactivity (b) capability of looking at multiple targets (c) background signal effect from non-specific adsorption to rea- gents and surfaces and (d) Increased specificity and sensitivity from additional proofreading.
References
7. Brenner S., Jacob F., Meselson M. An unstable intermediate carrying information from genes to ribosomes for protein. Nature 190:576-581.
35. Omenn GS, States DJ., Adamski M., Blackwell TW., Menon R., Hermjakob H., Apweiler R., Haab BB., Simpson RJ., Eddes JS et al., Overview of the HUPO Plasma Proteome Project: results from the pilot phase with. Proteomics 13:3226-45.
53. Ronquist G. and Hedstrom M - Restoration of detergent-inactivated adenosine triphosphatase activity of human. - Biochim Biophys Acta. 1977 Aug 11;483(2):483-6.:- 483-6.
54. Ronquist G., Brody I., Gottfries A., Stegmayr B - An Mg2+ and Ca2+- stimulated adenosine triphosphatase in human prostatic. - Andrologia. 1978 Nov-Dec;10(6):427-33.:- 427-33.
55. Ronquist G. and Brody I. - The prostasome: its secretion and function in man. - Biochim Biophys Acta. 1985 Sep 9;822(2):203-18.:- 203-18.
56. Johnstone RM., Adam M., Hammond JR., Orr L., and Turbide C - Vesicle formation during reticulocyte maturation. Association of plasma membrane. - J Biol Chem. 1987 Jul 5;262(19):9412-20.:- 9412-20.
59. Ronquist G. - Prostasomes are mediators of intercellular communication:
from basic research to. J Intern Med. 2012;271(4):400-13.
67. Paone JF., Waalkes TP., Baker RR., and Shaper JH. Serum UDP- galactosyl transferase as a potential biomarker for breast carcinoma. J Surg Oncol. 1980;15(1):59-66.
3 80. Jonsson U., Fagerstam L., Ivarsson B., Johnsson B., Karlsson R., Lundh K., Lofas S., Persson B., Roos H., Ronnberg I., et al., Real-time biospecific interaction analysis using surface plasmon resonance and a sensor chip technology. Biotechniques. 1991;11(5):620-7.
87. Leenaars M. and Hendriksen CF. Critical steps in the production of polyclonal and monoclonal antibodies: evaulation and recommendation. Ilar J. 2005;46(3):269-79.
90. Winter G., Griffiths AD., Hawkins RE. and Hoogenboom HR. Making antibodies by phage display technology. Annu Rev Immunol. 1994;12(0732- 0582 (Print)):433-55
92. Muyldermans S. Nanobodies: natural single-domain antibodies. - Annu Rev Biochem. 2013;82(1545-4509 (Electronic)):775-97.
96. Nygren PA. Alternative binding proteins: affibody binding proteins developed from a small. FEBS 2008;275(11):2668-76.
110. Burnette WN. Western blotting": electrophoretic transfer of proteins from sodium dodecyl. Analytical biochemistry. 1981;112(0003-2697 (Print)):195-203
117. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature. 1970;15(227 (5259)):680-5.
120. Ornstein L. DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY. Ann N Y Acad Sci. 1964;121(- 0077-8923 (Print)):321-49.
121. Davis BJ. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci 1964;1964(121):404-27.
124. Görg A., Obermaier C., Boguth G., Harder A., Scheibe B., Wildgruber R., and Weiss W. The current state of two-dimensional electrophoresis with immobilized pH. Electrophoresis. 2000;21( 0173-0835 (Print)):1037-53.
125. Corthals GL., Wasinger VC., Hochstrasser DF. and Sanchez JC.
The dynamic range of protein expression: a challenge for proteomic research. Electrophoresis. 2000;6(0173-0835 (Print)):1104-15.
126. Edman P. A method for the determination of amino acid sequence in peptides. Arch Biochem. 1949;22(0096-9621 (Print)):475.
128. Wide L., Bennich H. and Johansson S. G. Diagnosis of allergy by an in-vitro test for allergen antibodies. Lancet. 1967;25(7526):1105-7.
130. Uotila M., Ruoslahti E. and Engvall E. Two-site sandwich enzyme immunoassay with monoclonal antibodies to human. Journal of immunological methods. 1981;42(0022-1759 (Print)):11-5.
135. Wingren C. and Borrebaeck CA. Antibody-based microarrays.
Methods Mol Biol. 2009;509(1064-3745 (Print)):57-84.
137. McHugh TM., Wang YJ., Chong HO., Blackwood LL. and Stites DP. Development of a microsphere-based fluorescent immunoassay and its comparison to an enzyme immunoassay for the detection of antibodies to three antigen preparations from Candida albicans. J immunolo Methods 1989;116(0022-1759 (Print)):213-9.
139. de Jager W., te Velthuis H., Prakken BJ., Kuis W. and Rijkers GT Simultaneous Detection of 15 Human Cytokines in a Single Sample of
4
Stimulated Peripheral Blood Mononuclear Cells. Clin Diagn Lab Immunol.
2003;1(1071-412X (Print)):133-9.
143. Xu XH., Jeffers RB., Gao J. and Logan B Novel solution-phase immunoassays for molecular analysis of tumor markers. Analyst. 2001;8(
0003-2654 (Print)):1285-92.