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Molecular Biotechnology Programme Uppsala University School of Engineering

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Molecular Biotechnology Programme

Uppsala University School of Engineering

UPTEC X 08 013 Date of issue 2008-02

Author

Per Jungnelius

Title (English)

Asymmetrical flow field-flow fractionation system parameters and its use on optimized quantitative antibody separation

Title (Swedish)

Abstract

Method development parameters for the novel separation method asymmetrical flow field- flow fractionation were investigated and evaluated using design of experiments methodology.

An optimized method for separation of immunoglobulin G aggregates was developed and results were correlated with size-exclusion high performance liquid chromatography, sedimentation velocity analytical ultracentrifugation, native polyacrylamide gel- electrophoresis and dynamic light scattering.

Keywords

A4F, FFF, method development, IgG, SE-HPLC, SV-AUC, native PAGE, DLS, DoE.

Supervisors

Michael Wouda, M.Sc.

Analytical Sciences, Biovitrum AB, Stockholm

Scientific reviewer

Prof. Karin Caldwell

Dept. of Surface Biotechnology, Uppsala University

Project name Sponsors

Language

English

Security

ISSN 1401-2138 Classification

Supplementary bibliographical information

Pages

58

Biology Education Centre Biomedical Center Husargatan 3 Uppsala

Box 592 S-75124 Uppsala Tel +46 (0)18 4710000 Fax +46 (0)18 555217

References

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