Molecular Biotechnology Programme
Uppsala University School of Engineering
UPTEC X 14 001 Date of issue 2014-02
Author
Johan Björkesten
Title (English)
Development and evaluation of procedures and reagents for extraction of proteins from dried blood spots for analysis using
Proseek
Title (Swedish) Abstract
A method for extraction of proteins from dried blood spots (DBS) for analysis using Proseek is developed and evaluated. DBS, as sample format, possesses a number of desirable advantages over for example plasma samples. These advantages include for example minimal patient invasiveness, sampling simplicity and non regulated sample transportation. Highly reproducible quantitative detection of 92 proteins is demonstrated from a 1.2 mm in diameter DBS disk. The DBS inter spot analysis precision (7% coefficient of variance) is comparable to plasma inter assay precision (6% coefficient of variance). Coefficient of variance is the ratio between standard deviation to mean value for the analysed replicates. Proseek analysis of DBS could possibly reveal a unique opportunity to examine health related issues in extremely premature infants hopefully resulting in increased survival rates in the future.
Keywords
DBS, dried blood spots, PEA, Proseek, Proseek Multiplex, qPCR, Protein detection, Olink Supervisors
Dr. Mats Gullberg
Olink Bioscience Scientific reviewer
Dr. Masood Kamali-Moghaddam
Uppsala University
Project name Sponsors
Language
English
Security
ISSN 1401-2138 Classification
Supplementary bibliographical information Pages
64
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