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UPTEC X 11 004

Date of issue 2011-02

Author

Hanna Garbergs

Title (English)

Extraction of therapeutic proteins from dried blood spots and their analysis on Gyrolab

Title (Swedish)

Abstract

A method for extraction of therapeutic proteins from dried blood spots (DBS) followed by quantification on GyrolabTM has been developed. The method makes it possible to measure the concentration of the analyte in the range 100-6000 ng/mL. The procedure can generate full analytical information from 15 µL blood originally sampled from a subject. The modest sample requirements allows for sampling a full pre-clinical pharmacokinetic profile from a single mouse. This may allow for reduced usage of animals during preclinical development of new therapeutic proteins in accordance with the 3R’s, replace, refine and reduce.

Keywords

Dried blood spots, immunoassays, therapeutic proteins, monoclonal antibodies, Gyrolab.

Supervisors

Mats Inganäs

Gyros AB

Scientific reviewer

Ola Söderberg

Uppsala University

Project name Sponsors

Language

English

Security

None

ISSN 1401-2138 Classification

Supplementary bibliographical information Pages

49

Biology Education Centre Biomedical Center Husargatan 3 Uppsala Box 592 S-75124 Uppsala Tel +46 (0)18 4710000 Fax +46 (0)18 471 4687

References

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