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Molecular Biotechnology Programme Uppsala University School of Engineering

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Molecular Biotechnology Programme

Uppsala University School of Engineering

UPTEC X 09 021 Date of issue 2009-07 Author

Kristina Råsbo

Title (English)

Study of the autoimmune B cell response in complement receptor deficient mice

Title (Swedish) Abstract

Collagen-induced arthritis (CIA) is an animal model used to study the immune mechanisms behind the human autoimmune disease rheumatoid arthritis (RA). In this study wild type (WT) and complement receptor 1 and 2 (CR1/2)-deficient DBA/1 mice have been examined in order to investigate the role of complement receptors in the regulation of self-reactive B cells in CIA. The mice were immunized with collagen type II (CII) in adjuvant and the B cells from the spleen and the lymph nodes were analyzed using ELISPOT. Our results indicate that self-tolerance to CII in the spleen breaks earlier in CR1/2-deficient mice than in WT mice.

Additionally, we have seen that naïve DBA/1 mice have CII-reactive B cells present in the spleen prior immunization.

Keywords

Complement receptors, B cells, mice, collagen-induced arthritis Supervisors

Sandra Kleinau and Cecilia Carnrot Uppsala universitet

Scientific reviewer

Birgitta Heyman Uppsala universitet

Project name Sponsors

Language

English

Security

ISSN 1401-2138 Classification

Supplementary bibliographical information

Pages

26

Biology Education Centre Biomedical Center Husargatan 3 Uppsala

Box 592 S-75124 Uppsala Tel +46 (0)18 4710000 Fax +46 (0)18 471 4687

References

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