APPENDIX 2

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APPENDIX 2

Table 1: Dilution of known concentration of human IgA for standard curve on all plates

Standard curve for all plates Dilution Concentration

of standard IgA Procedure

Pre-step 1 12000ng/mL 10µL human IgA + 882 µL PBT Pre-step 2 _{2000 ng/mL} 100 µL of step 1 and 500 µL PBT Dilution 1 1000 ng/mL 200 µL of above and 200 µL PBT Dilution 2 _{500 ng/mL} _{200 µL of above and 200 µL PBT} Dilution 3 250 ng/mL 200 µL of above and 200 µL PBT Dilution 4 _{125 ng/mL} _{200 µL of above and 200 µL PBT} Dilution 5 62.5 ng/mL 200 µL of above and 200 µL PBT Dilution 6 _{31.25 ng/mL} _{200 µL of above and 200 µL PBT} Dilution 7 15.6 ng/mL 200 µL of above and 200 µL PBT Dilution 8 _{7.8 ng/mL} _{200 µL of above and 200 µL PBT}

- Standard IgA = Human IgA from colostrum (Sigma, artnr:I-1010)

Table 2: Dilution of known concentration of human IgA for reference standard curve

Standard curve for reference Dilution Concentration

of standard IgA Procedure

Pre-step 1 _12000ng/mL _{10µL human IgA + 882 µL PBT} Pre-step 2 2000 ng/mL 100 µL of step 1 and 500 µL PBT Dilution 1 _{1000 ng/mL} _{200 µL of above and 200 µL PBT} Dilution 2 500 ng/mL 200 µL of above and 200 µL PBT Dilution 3 _{375 ng/mL} _{300 µL of above and 100 µL PBT} Dilution 4 250 ng/mL 200 µL of dilution 2 and 200 µL PBT Dilution 5 _{187,5 ng/mL} _{200 µL of dilution 3 and 200 µL PBT} Dilution 6 125 ng/mL 200 µL of dilution 4 and 200 µL PBT Dilution 7 _{93,75 ng/mL} _{200 µL of dilution 5 and 200 µL PBT} Dilution 8 62,5 ng/mL 200 µL of dilution 6 and 200 µL PBT Dilution 9 _{46,88 ng/mL} _{200 µL of dilution 7 and 200 µL PBT} Dilution 10 31,25 ng/mL 200 µL of dilution 8 and 200 µL PBT Dilution 11 _{23,44 ng/mL} _{200 µL of dilution 9 and 200 µL PBT} Dilution 12 15,6 ng/mL 200 µL of dilution 10 and 200 µL PBT Dilution 13 _{11,71 ng/mL} _{200 µL of dilution 11 and 200 µL PBT} Dilution 14 7,8 ng/mL 200 µL of dilution 12 and 200 µL PBT Dilution 15 _{5,86 ng/mL} _{200 µL of dilution 13 and 200 µL PBT} Dilution 16 3,9 ng/mL 200 µL of dilution 14 and 200 µL PBT Dilution 17 _{2,93 ng/mL} _{200 µL of dilution 15 and 200 µL PBT} Dilution 18 _{1,95 ng/mL} _{200 µL of dilution 16 and 200 µL PBT}