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Molecular Biotechnology Programme Uppsala University School of Engineering

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Molecular Biotechnology Programme

Uppsala University School of Engineering

UPTEC X 07 047 Date of issue 2007-09

Author

Anna Engström

Title (English)

Screening for anti-virulence compounds against Escherichia coli and investigation of triclosan resistance development in

Staphylococcus aureus

Title (Swedish) Abstract

Two strategies to find treatments against nosocomial infections were investigated in this degree project. Anti-virulence is a new concept for antibacterial therapy. Anti-virulence drugs render pathogenic bacteria sensitive to the complement system so that they can be cleared from the body in a natural way. In the first part of the project a high-throughput screening of 14 727 synthetic compounds was performed on Escherichia coli. The other part was involved in the development of a novel antibiotic drug, a FabI inhibitor. The aim was to generate a triclosan resistant Staphylococcus aureus strain for cross-resistance studies with triclosan and to investigate the underlying resistance mechanism of the obtained clones as well as of an S.

aureus strain resistant to the FabI inhibitor MUT021142-00-B.

Keywords

Nosocomial infections, anti-virulence, complement system, high-throughput screening, Escherichia coli, antibiotics, FabI, Staphylococcus aureus, triclosan

Supervisors

Coralie Soulama-Mouzé

Mutabilis SA, Romainville, France Scientific reviewer

Diarmaid Hughes

Department of Cell and Molecular Biology, Uppsala University

Project name Sponsors

Language

English

Security

Secret until 2012-01-29

ISSN 1401-2138 Classification

Supplementary bibliographical information Pages

46

Biology Education Centre Biomedical Center Husargatan 3 Uppsala Box 592 S-75124 Uppsala Tel +46 (0)18 4710000 Fax +46 (0)18 555217

References

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