Linköping Studies in Science and Technology Dissertation No. 1376
Zinc Oxide Nanostructure Based Electrochemical Sensors
and Drug Delivery to Intracellular Environments
Muhammad Asif
Linköping University INSTITUTE OF TECHNOLOGY Human Adipocyte
Linköping Studies in Science and Technology Dissertation No. 1376
Zinc Oxide Nanostructure Based Electrochemical Sensors
and Drug Delivery to Intracellular Environments
Muhammad Asif
Linköping University INSTITUTE OF TECHNOLOGY Human Adipocyte
Linköping Studies in Science and Technology Dissertation No. 1376
Zinc Oxide Nanostructure Based Electrochemical Sensors
and Drug Delivery to Intracellular Environments
Muhammad Asif
Linköping University INSTITUTE OF TECHNOLOGY Human Adipocyte Muhammad Asif
Zinc Oxide Nanostructur
e Based Electr
ochemical S
ensors and Drug Deli
very t o Intr ac ellular En vir onments Link öping 2011
Linköping University, Doctoral Dissertation
This doctoral dissertation described the developement of a new type of intracellular electrochemcal biosensors and photodynamic therapy based on funtionalized zinc oxide nanorods. The aim of this study is to highlight recent developments in materials and techniques for electrochemical biosensing, photodynamic therapy, design, operation, and fabrication. The sensors in this study were used to detect and monitor real changes of metal ions and glucose across human fat cells and frog cells using changes in the electrochemical potential at the interface to the intracellular microenvironments. Moreover the zinc oxide nanorods were suggested and used as efficient carrier of photosensitizers for cancerous cell treatment.
This thesis presents intracellular studies on, metal ions, glucose and photodynamic therapy by using ZnO nanorods grown on the tip of a borosilicate glass capillary (0.7 µm in diameter) with the aim to produce, proto-type electrochemical intracellular biosensors and efficient carrier of photosensitizers for cancerous cell treatment. Breast cancer cells were used to study the catalytic effect of ZnO for treatment. The grown ZnO nanorods were conjugated with protoporphyrin dimethyl ester (PPDME), which absorbs the light emitted by the ZnO nanorods and cause the cytotoxicity which appears to involve the generation of reactive singlet oxygen inside the cell.
Muhammad Asif carried out his postgraduate studies at the Division of Physical Electronics and Nanotechnology, Department of Science and Technology (ITN), Linköping University, Sweden in 2011. He completed his Licentiate studies at the same department, Linköping University, Sweden in 2009.
Linköping Studies in Science and Technology Dissertation No. 1376
Zinc Oxide Nanostructure Based Electrochemical Sensors
and Drug Delivery to Intracellular Environments
Muhammad Asif
Linköping University INSTITUTE OF TECHNOLOGY Human Adipocyte
Linköping Studies in Science and Technology Dissertation No. 1376
Zinc Oxide Nanostructure Based Electrochemical Sensors
and Drug Delivery to Intracellular Environments
Muhammad Asif
Linköping University INSTITUTE OF TECHNOLOGY Human Adipocyte
Linköping Studies in Science and Technology Dissertation No. 1376
Zinc Oxide Nanostructure Based Electrochemical Sensors
and Drug Delivery to Intracellular Environments
Muhammad Asif
Linköping University INSTITUTE OF TECHNOLOGY Human Adipocyte Muhammad Asif
Zinc Oxide Nanostructur
e Based Electr
ochemical S
ensors and Drug Deli
very t o Intr ac ellular En vir onments Link öping 2011
Linköping University, Doctoral Dissertation
This doctoral dissertation described the developement of a new type of intracellular electrochemcal biosensors and photodynamic therapy based on funtionalized zinc oxide nanorods. The aim of this study is to highlight recent developments in materials and techniques for electrochemical biosensing, photodynamic therapy, design, operation, and fabrication. The sensors in this study were used to detect and monitor real changes of metal ions and glucose across human fat cells and frog cells using changes in the electrochemical potential at the interface to the intracellular microenvironments. Moreover the zinc oxide nanorods were suggested and used as efficient carrier of photosensitizers for cancerous cell treatment.
This thesis presents intracellular studies on, metal ions, glucose and photodynamic therapy by using ZnO nanorods grown on the tip of a borosilicate glass capillary (0.7 µm in diameter) with the aim to produce, proto-type electrochemical intracellular biosensors and efficient carrier of photosensitizers for cancerous cell treatment. Breast cancer cells were used to study the catalytic effect of ZnO for treatment. The grown ZnO nanorods were conjugated with protoporphyrin dimethyl ester (PPDME), which absorbs the light emitted by the ZnO nanorods and cause the cytotoxicity which appears to involve the generation of reactive singlet oxygen inside the cell.
Muhammad Asif carried out his postgraduate studies at the Division of Physical Electronics and Nanotechnology, Department of Science and Technology (ITN), Linköping University, Sweden in 2011. He completed his Licentiate studies at the same department, Linköping University, Sweden in 2009.
