Molecular Biotechnology Programme
Uppsala University School of Engineering
UPTEC X 05 025 Date of issue 2005-04 Author
Maria Kärrlander
Title (English)
Purification and characterization of polyclonal antibodies against tumour suppressor protein APC
Title (Swedish) Abstract
An important part in the fight against cancer is finding high affinity reagents for proteomics research. One protein extensively studied is the tumour suppressor adenomatous polyposis coli or APC, involved in colon cancer. In this project polyclonal antibodies against APC from immunized rabbits were purified. The antibodies were found to be useful in western blot analysis, immunoprecipitation of native APC and immunocytochemistry.
Keywords
Antibody purification, APC, Biosensor analysis, proteomics
Supervisors
Eduardo Nice
Ludwig Institute for Cancer Research, Melbourne
Scientific reviewer
Carl-Henrik Heldin
Ludwig Institute for Cancer Research, Uppsala
Project name Sponsors
Language
English
Security
ISSN 1401-2138 Classification
Supplementary bibliographical information Pages
28
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