• No results found

Molecular Biotechnology Programme Uppsala University School of Engineering

N/A
N/A
Info
Download
Protected

Academic year: 2022

Share "Molecular Biotechnology Programme Uppsala University School of Engineering"

Copied!
1
0
0

Loading.... (view fulltext now)

Download now ( 1 Page )

Full text

(1)

Molecular Biotechnology Programme

Uppsala University School of Engineering

UPTEC X 07 035 Date of issue 2007-06

Author

Daniel Kamsund

Title (English)

Synthetic biology – characterizing promoters and evaluating the methods

Title (Swedish) Abstract

Synthetic biology is a new genetic engineering approach built on: (i) a complexity hierarchy of parts (e.g. promoters), devices and systems, and (ii) standardization. I have evaluated fluorescence imaging, microscopy imaging and Northern blotting as methods to characterize and quantify the strength of individual promoters. Three different promoters were assembled with fluorescent protein constructs, using the BioBrick system, and characterized.

Quantifications revealed that the promoters behaved like the theoretical models predicted and fluorescence imaging was found to be the most robust quantification method.

Keywords

Synthetic biology, BioBrick, genetic engineering, promoters, promoter characterization, fluorescence imaging, fluorescence microscopy, Northern blotting.

Supervisors

Prof. Peter Lindblad Dr. Thorsten Heidorn

Department of Photochemistry and Molecular Science, Uppsala University Scientific reviewer

Assoc. Prof. Lage Cerenius

Department of Physiology and Developmental Biology, Uppsala University

Project name Sponsors

Language

English

Security

ISSN 1401-2138

Classification

Supplementary bibliographical information Pages

38

Biology Education Centre Biomedical Center

Husargatan 3 Uppsala Box 592 S-75124 Uppsala Tel +46 (0)18 4710000 Fax +46 (0)18 555217

References

Download now ( PDF - 1 Page - 270.27 KB )

Related documents

Molecular Biotechnology Programme Uppsala University School of Engineering

Moreover, by applying a molecular clock, it is proposed that the Domestic cat lineage has experienced several expansions representing domestication and/or breeding

Molecular Biotechnology Programme Uppsala University School of Engineering

It showed large dynamic range, no measureable non-specific binding and high sensitivity (with linear range around 0.1 – 10 µg/ml depending on the proteins). The method

Molecular Biotechnology Programme Uppsala University School of Engineering

Escherichia coli, 23S rRNA, Post-transcriptional modification, In vitro, Peptidyl transferase centre, domain V, Methylation, Pseudouridine, Reverse

Molecular Biotechnology Programme Uppsala University School of Engineering

The fibrinolytic enzyme tissue type plasminogen activator (t-PA) is released by the vascular endothelium to limit the growth of blood clots and ultimately prevent the occurrence of

Molecular Biotechnology Programme Uppsala University School of Engineering

investigate the presence of P4 in the brain, in neuromuscular junctions and in adrenal glands, the colocation between P4 and VAChT respectively P4 and VMAT and the possibility that P4

Molecular Biotechnology Programme Uppsala University School of Engineering

The information obtained by using these algorithms could contribute to the development of new drugs by generating new ligands that target specific high-energy , unfavorable

Molecular Biotechnology Programme Uppsala University School of Engineering

Results from the project shows that when multiplexing there are differences in measured IgE- levels between an existing singleplex method and the multiplex prototype. The project also

Molecular Biotechnology Programme Uppsala University School of Engineering

A method for measuring cell concentration and identity based on flow cytometry (FCM) and fluorescent marking is developed and subsequently compared with traditional plating based