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Molecular Biotechnology Programme Uppsala University School of Engineering

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Molecular Biotechnology Programme

Uppsala University School of Engineering

UPTEC X 05 030 Date of issue 2005-05 Author

Jesper Gantelius

Title (English)

Desensitisation and internalisation of wildtype and mutant variants of the hY2 receptor

Title (Swedish) Abstract

Wildtype and mutant variants of the Neuropeptide Y receptor Y2 were cloned in HEK293 cells and investigated upon desensitisation and internalisation when stimulated with the ligands NPY, PYY and PYY3-36. It was shown that the wildtype as well as the mutants exhibited low degre of internalisation, and that the internalisation of the wildtype increased linearly, even at saturating concentrations of ligand. A surprising effect suggesting that Adenylate Cyclase may internalise together with the receptor has also been observed.

Keywords

NPY, PYY, PYY3-36 ,hY2, GPCRs, Internalisation, Desensitisation, Adenylate Cyclase

Supervisors

Ingrid Lundell

Department of Neuroscience, Uppsala University

Scientific reviewer

Prof. Dan Larhammar

Department of Neuroscience, Uppsala University

Project name Sponsors

Language

English

Security

ISSN 1401-2138 Classification

Supplementary bibliographical information Pages

24

Biology Education Centre Biomedical Center Husargatan 3 Uppsala Box 592 S-75124 Uppsala Tel +46 (0)18 4710000 Fax +46 (0)18 555217

References

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