• No results found

Neuropeptide Y receptor Y2 site-directed mutagenesis

N/A
N/A
Info
Download
Protected

Academic year: 2022

Share "Neuropeptide Y receptor Y2 site-directed mutagenesis"

Copied!
1
0
0

Loading.... (view fulltext now)

Download now ( 1 Page )

Full text

(1)

Neuropeptide Y receptor Y2 site-directed mutagenesis Jasna Pruner

During the past few years, the structural biology of G-protein-coupled-receptors has seen remarkable advances and crystallographic data for a number of receptors became available. This data offers valuable clues for constructing templates for computational 3D model building of receptors with unresolved structures such as for instance, the receptors in the neuropeptide Y family. Neuropeptide Y2 receptor is an attractive pharmacological target for anti-obesity medications due to its appetite suppressing properties in response to peptide YY

3-36

. Experimental attempts to design a peptidomimetic based on peptide YY

3-36

structure require a detailed structure of neuropeptide Y2 receptor. This degree project is a part of research project aiming to determine the properties of the Y2 receptor ligand-binding network by an integrated approach combining computational modeling and site-directed mutagenesis. Two docking solutions of the human neuropeptide Y to the Y2 receptor have revealed ligand binding positions that became the candidates for site-directed mutagenesis. The aim of this degree project is to introduce mutations Y

1.39

A, Q

6.55

L, Q

3.32

A, Q

3.32

L, H

7.39

A and H

7.39

L into the plasmids carrying the Y2 coding region. Preliminary tests indicate a binding loss in receptors carrying amino acid substitutions Q

6.55

L, Q

3.32

A, H

7.39

A and H

7.39

L. These results are consistent with the docking solutions we propose for the Y2 receptor.

Degree project in Biology, Master of Science (1 year), 2011 Examensarbete i biologi 15hp till magisterexamen, 2011

Biology Education Centre and Department of Neuroscience, Unit for Pharmacology, Uppsala University

Supervisor: Dan Larhammar

References

Download now ( PDF - 1 Page - 7.86 KB )

Related documents

Peroxidases: A computational study of ligand binding and enzyme kinetics

One directs the indole substituent to- wards the distal cavity (IS-modes) and the other in- stead directs the sidechain in that direction (SC- modes). The same modes reappearing

The Design of Prosperity research project

The Design of Prosperity Event On November 7–8 2006, The Swedish School of Textiles and the Göteborg University School of Business, Economics and Law brought together a unique

The elusive ligand complexes of the DWARF14 strigolactone receptor

Electron density maps calculated from the structure show only very weak density in the ligand-binding cavity (PDB id 5dj5) and there are no significant confor- mational

1 ≤ x2+ y2 ≤ 4 , x ≥ y ≥ 0

[r]

Ledvis subtraktion ger ekvationen x2− y2 = 2(y − x), som efter tillämpning av konjugatregeln kan skrivas x2− y2 = 2(y − x

Vår första strategi för att bevisa denna olikhet är att försöka skriva om vänsterledet här som en kvadrat, eller en summa av kvadrater, eller en summa av på annat sätt

”The Culture Cube – Degree Project”

Det som är viktigt är dels en tydlig närvaro av lärare eller andra vuxna inom skolan, ytor där lärare och barn kan umgås tillsammans utanför lektionstid, samt placering av

x2+ y2− 1 ≥ 0 och 4 − x2− y2 ≥ 0

Eftersom uttrycket x 2 + y 2 ¨ar lika med kvadraten p˚a avst˚andet fr˚an punkten (x, y) till origo s˚a ger ( ∗) omr˚adet mellan cirklar- na med radie 1 och 2 centrerade kring

Rätt svarsalternativ: d a Går ej b 6x x 1 3 y2 y C1 c 6x x 3 y2 y C1 d 6x x 1 3 y2 y C1 e Inget av a till d

Ökningen av antalet kaniner k t per tidsenhet är proportionell med Α mot det aktuella antalet kaniner och skillnaden mellan maximala antalet och det aktuella antalet.. Vattnet i