Degree Project in Molecular Biotechnology
Masters Programme in Molecular Biotechnology Engineering, Uppsala University School of Engineering
UPTEC X 15 009
Date of issue 2015-06 AuthorErica Nyberg
Title (English)
Epigenetic reprogramming of somatic embryos from Norway spruce cultured in bioreactors
Title (Swedish)
Abstract
Epigenetics is defined as stable heritable changes in gene expression that are not caused by changes in DNA sequence. The most predominant epigenetic regulation is methylation of cytosines. Methylation of cytosines has been shown to regulate several traits in both mammals and plants. One such trait is cold hardiness in plants, believed to be established in part already during embryogenesis. In this study, somatic embryos of Norway spruce were cultured in different temperatures to cause change in the epigenetic memory suspected to regulate genes involved in cold hardiness. Protocols for analysing the methylation changes using bisulfite conversion were established.
Keywords
Epigenetics, Norway spruce, bisulfite conversion, somatic embryogenesis, bioreactors.
methylation
Supervisors
Ines Ezcurra Ulrika Egertsdotter Royal Institute of Technology SweTree Technologies Scientific reviewer
David Clapham SweTree Technologies
Project name Sponsors
Language
English Security Secret until 2025-06
ISSN Classification
Supplementary bibliographical information Pages
32
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