Degree Project in Molecular Biotechnology
Masters Programme in Molecular Biotechnology Engineering, Uppsala University School of Engineering
UPTEC X 15 033
Date of issue 2015-10Author
Agnes Andersson Svärd
Title (English)
Peripheral blood cell HLA class II gene expression in children at genetic risk for type 1 diabetes and coeliac disease
Title (Swedish) Abstract
Differential expression of HLA-DQ heterodimers on a blood mononuclear cell subset is associated with organ-specific autoimmune disease. HLA-DQ genotypes on chromosome 6 are strongly related to increased risk of developing autoimmune diseases such as type 1 diabetes, and coeliac disease. Peripheral blood subsets from children at increased genetic risk for type 1 diabetes (HLA-DQ2/8; DQ8/8; DQ8/X (X is not 6.2); DQ2/2) or coeliac disease (HLA-DQ2/2; DQ2/8) with and without islet cell autoantibody markers (GADA, IAA, IA-2A, and ZnT8 (W, R, Q)A) were investigated for HLA-DQA1, B1, A2 and B2 gene expression.
High HLA-DQ cell surface immunofluorescence was observed in B cells and CD14+CD16- APC. HLA-DQA1 is expressed in CD14+CD16- APCs and B cells. The results indicate that RQ of HLA-DQA1 tended to be lower in CD14+CD16- APCs in subjects with more than two islet autoantibodies.
Keywords
Type 1 diabetes, islet autoantibodies, HLA, HLA-DQ, B cells, CD14+CD16- APCs, flow cytometry, gene expression
Supervisors
Professor Åke Lernmark
Department of diabetes and coeliac disease, Lund University
Scientific reviewer
Professor Ulf Gyllensten
Human genomics and molecular epidemiology, Uppsala University
Project name Sponsors
Language
English
Security
ISSN 1401-2138 Classification
Supplementary bibliographical information Pages
35
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