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Acta Odontologica Scandinavica
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Salivary fluoride concentration and retention
after rinsing with 0.05 and 0.2% sodium fluoride
(NaF) compared with a new high F rinse containing
0.32% NaF
Kerstin Larsson , Alexander Stime , Lisa Hansen , Dowen Birkhed & Dan
Ericson
To cite this article: Kerstin Larsson , Alexander Stime , Lisa Hansen , Dowen Birkhed & Dan Ericson (2020): Salivary fluoride concentration and retention after rinsing with 0.05 and 0.2% sodium fluoride (NaF) compared with a new high F rinse containing 0.32% NaF, Acta Odontologica Scandinavica, DOI: 10.1080/00016357.2020.1800085
To link to this article: https://doi.org/10.1080/00016357.2020.1800085
© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of Acta Odontologica Scandinavica Society.
Published online: 30 Jul 2020.
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ORIGINAL ARTICLE
Salivary fluoride concentration and retention after rinsing with 0.05 and 0.2%
sodium fluoride (NaF) compared with a new high F rinse containing 0.32% NaF
Kerstin Larssona, Alexander Stimeb, Lisa Hansenc, Dowen Birkheddand Dan Ericsona a
Department of Cariology, Faculty of Odontology, Malm€o University, Malm€o, Sweden;bKungstandl€akarna AB, Stockholm, Sweden;cLycko Pers v€ag, Trelleborg, Sweden;dFersens v€ag, Malm€o, Sweden
ABSTRACT
Objective: To compare salivary fluoride (F) concentration and F retention after rinsing with a new 0.32% sodium fluoride (NaF) rinse and conventional 0.05 and 0.2% NaF rinses.
Methods: Seventeen subjects (aged 22–26 years), with normal salivary secretion rates, participated in a double blind, cross-over study. In three separate sessions with a minimum washout period of 48 h, they rinsed for 1 min with 10 ml of 0.05, 0.2 or 0.32% NaF mouthrinse. Unstimulated whole saliva was collected before (baseline: 0 min) and after 1, 3, 5, 10, 20, 30, 45 and 60 min. The F concentration was plotted against time, and the area under the curve (AUC) calculated. Salivary F concentration and F retention for the three mouthrinses were compared by a randomized block test, followed by Tukey’s test and a paired 2-tailed test.
Results: There was a clear dose–response for AUC 3–60 min; 0.32% > 0.2% > 0.05% (p < .05). The mean F retention was 0.25 mg for 0.05% NaF, 0.86 mg F for 0.2% Na and 1.31 mg F for 0.32% NaF, (p < .05). Conclusions: The higher salivary F concentration over time and the higher F retention after rinsing with an 0.32% NaF solution suggests a potential application in prevention of caries and dental erosion.
ARTICLE HISTORY Received 2 March 2020 Revised 21 May 2020 Accepted 19 July 2020 KEYWORDS Fluoride; mouthrinse solutions; retention; unstimulated whole saliva
Introduction
There is a dose–response relationship between the fluoride (F) concentration in toothpaste and the caries preventive effect [1]. Thus, the more F, the better effect. Higher F con-centrations in the paste (5000 vs. 1450 ppm F) give higher levels of F in the oral biofilm [2,3], as well as in the oral mucosa and saliva [4]. There is strong evidence to support the use of high F toothpastes (5000 ppm) in teenagers at greater risk for caries, e.g. those brushing only once a day [5,6]. Regular use of dentifrices containing 5000 ppm F may also be of special interest for prevention of root caries [7,8]. However, in younger children the F concentration must be balanced against the risk of dental fluorosis.
In many countries, F mouthrinse solutions are recom-mended as a supplement to F toothpaste for patients at risk for caries and dental erosion. The most commonly used con-centration for adults and the elderly is 0.2% sodium fluoride (NaF) (900 ppm F) for daily rinsing [9,10]. Lower concentra-tions (0.05% NaF; 225 ppm F) however, are recommended for children<12 years and this is the standard concentration in many countries [11,12].
Post-brushing rinsing for control of dental caries was recently discussed at a consensus meeting of experts from Europe and USA, as to what advice should be given to patients [13]. One of the statements of the group was that
‘mouthrinses containing F can be used after brushing with F toothpaste’. It could be argued that the minimum concentra-tion of NaF soluconcentra-tion to be used as a post-brushing rinse should be 0.2% – in order not to dilute the F concentration after brushing with 0.32% NaF toothpaste. This is in accord-ance with the study by Mystikos et al. [14] showing that post-brushing mouthrinse solutions exert a ‘wash-out’ effect if the concentration of F is too low, i.e.0.05% NaF.
With respect to the role of F in prevention of erosion, Huysman et al. [15] concluded that‘solutions and rinses with high F concentrations’ are most promising. In this context, it was of interest to evaluate a recently introduced high F mouthrinse solution, containing 0.32% NaF (1450 ppm F), i.e. the same concentration as in most F toothpastes today.
The aim of the present study was to compare salivary F concentration in the hour following rinsing with 0.05, 0.2 and 0.32% NaF and also to measure the retention, i.e. how much F is potentially swallowed.
Materials and methods Study subjects and design
Twenty healthy students, with subjectively normal salivary secretion, were recruited at the Faculty of Dentistry in Malm€o, Sweden, by means of a flyer. The volunteers were
CONTACTKerstin Larsson kerstin.m.larsson@mau.se Department of Cariology, Faculty of Odontology, Malm€o University, Carl Gustafs v€ag 34, Malm€o,
SE-214 21, Sweden
ß 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of Acta Odontologica Scandinavica Society.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
given verbal and written information before the study and gave written consent to participation. The study protocol was approved by the Ethics Committee, EPN, in Lund, Sweden, (DNR 2018/180). Three students chose not to enter the study.
The following inclusion criteria were applied: good gen-eral health and a minimum of 24 teeth, 6 in each quadrant, without extensive restorations. The criteria for exclusion were: gingivitis, periodontitis or active caries, orthodontic appliances or removable oral prostheses, pregnancy, and par-ticipation in any other study during the past month.
The design was a randomized, double-blind, cross-over study, with three treatment sessions.
Experimental protocol
Subjects were instructed not to brush their teeth after 11 p.m. the night before the test session. All subjects were asked to use a toothpaste containing 1450 ppm F (NaF), ‘Pepsodent Long Active Intensive Cleaning’ (Unilever, Solna, Sweden), which was provided free of charge.
At baseline, at the start of the first session, unstimulated saliva samples were collected for 5 min, to determine the secretion rate and to measure the baseline salivary F level.
At three sessions, separated by a minimum wash-out period of 48 h, the subjects were given one of three mouth-rinses with different F concentrations. The following products were used: 1) DentanVR
mint 0.05% NaF, containing 225 ppm F (Meda AB, Solna, Sweden), 2) DentanVR
mint 0.2% NaF, con-taining 900 ppm F, (Meda AB) and 3) Maximum Fluoride 0.32% NaF, Brilliant SmileTM, containing 1450 ppm F, (Brilliant Smile Sweden AB, Hisingsbacka, Sweden). The products were purchased from a pharmacy and the experimental supervi-sors and subjects were blinded to the contents. The table of contents, declared by the manufacturers, is presented in Table 1.
At each session, the subjects rinsed with 10 ml of the test solution for 1 min and then expectorated the saliva and mouthrinse solution into an empty pre-weighted plastic con-tainer (Container 25 ml 54 27PS þ Cap NAT, Sarstedt, Helsingborg) (0-min sample). Whole resting saliva samples were then collected at 1, 3, 5, 10, 20, 30, 45 and 60 min post-rinse, by passively letting saliva flow into a plastic con-tainer for one minute. The concon-tainer was then sealed with a tightly fitting screw lid. The first sample (0 min) was used to calculate the oral F retention.
During the test session, held in a quiet room, the subjects were not allowed to eat, drink or talk. The samples were stored in a freezer (20C) until analysed.
Fluoride determination
To determine the F concentration in the saliva samples, an ion-specific electrode, ISE, was used (PerfectIONTM F and Mettler ToledoTM, SevenCompact S220 Basic, pH/Ion benchtop metre, Mettler ToledoTM, Mettler-Toledo AB, Stockholm, Sweden). The ISE was calibrated according to the manufacturer’s instructions by using a series of F-containing standard solutions.
The available F concentrations of the three mouthrinses being tested were measured by the ISE, and were estimated to 243, 958 and 1464 ppm respectively. Ten milliliters of the rinse solution contained 2.4 mg, 9.6 mg and 14.6 mg F for each of the 0.05, 0.2 and 0.32% NaF rinses, respectively.
Prior to measurement, the saliva samples were heated to 25C in an incubator. For each saliva sample, an amount of 200ml was mixed with 20 ml Total Ionic Strength Adjustment Buffer (TISAB III, Mettler ToledoTM) and vortexed for 10 s before being placed on a plastic Petri dish, as a 100ml drop, in which the surface of the ISE was placed, in close contact with the solution, to measure the F concentration (ppm).
If the amount of saliva in a sample was less than 200ml, it was diluted 1:2, with deionized water, before adding TISAB III in proportions 1:10. The concentration was then measured as described above.
F retention was calculated as follows: the concentrations in the rinse solutions were measured by the ISE and the amount of F was determined by multiplying with the volume of 10 ml. The expectorated saliva was weighed (1 mg equals 1 ml), the concentration measured, and the amount of F cal-culated. This value was subtracted from the amount in the mouthrinse solution.
Data analysis
The primary efficacy variable was the integrated area under the curve (AUC) for F concentration in saliva as a function of time. The AUC for the three mouthrinses, 0.05%, 0.2% and 0.32% NaF, were compared by a randomized block design. If a significant difference was found, a post hoc test, Tukey’s test, followed.
The mean values for F retention of the three mouthrinses were calculated and compared by a paired 2-tailedt-test.
Table 1. Table of contents for the three mouthrinses, according to manufacturers’ product label.
0.05% NaF 0.2% NaF 0.32% NaF
Sodium fluoride 0.5 mg/ml Sodium fluoride 2 mg/ml Aqua hydrogenated starch hydrolysate
Xylitol 30 mg Xylitol 30 mg Glycerine
Methylparahydroxybenzoate Methylparahydroxybenzoate Potassium nitrate Peppermint oil Peppermint oil Propylene glycol
Water Water PEG-40-Hydrogenated castor oil
Other excipients Other excipients Sodium fluoride Sodium methylparaben Aroma
Menthol Sodium saccharin 2 K. LARSSON ET AL.
Results
Seventeen of the 20 volunteers participated in the test ses-sions: 13 women and 7 men, aged 22–26 years. In all, 358 samples were analysed, whereof two were diluted prior to measurement of F concentration. The mean resting salivary flow rate was 0.3 ml/min.
All samples which were too small in volume and therefore impossible to analyse, were excluded from the study. It was assumed that the change in F concentration over time in these subjects followed a linear pattern when calculating AUC. If a subject produced more than two salivary samples of very low volume for a certain mouthrinse, all samples of the said mouthrinse collected from this subject were excluded. The number of subjects on which the final calcula-tions for AUC 3–60 min were based, was 14 for the 0.05% solution, 12 for the 0.2% solution and 15 for the 0.32% solution.
The F concentration in the expectorated saliva was plot-ted against time from 1 to 60 min, and the area under the curve (AUC) was calculated for each of the three different mouthrinses. Clearance curves are shown in Figure 1(a,b) (both as logarithmic and non-logarithmic values). The inter-group differences are more pronounced during the first half of the clearance time period (Figure 1(a)).
As shown inFigure 2, Tukey’s test, after pairwise compari-son, disclosed a significant difference in AUC 3–60 min. This applied to all three concentrations of F rinse.
The median and mean values for the F retention for the three different mouthrinses are shown in the boxplot in Figure 3. As expected, the 0.32% NaF mouthrinse had the highest level of retention (1.31 mg), followed by 0.86 and 0.25 mg for the mouthrinses containing respectively 0.2% and 0.05% NaF (p<.05).
Discussion
A mouthrinse containing a high concentration of NaF (0.32%, 1450 ppm F) has recently been introduced to the Swedish market, as an over-the-counter (OTC) product. The aim of
this study was to compare this product with two commonly used OTC products available in several countries, containing either 0.2% (900 ppm F) or 0.05% NaF (225 ppm F). After a single, one minute rinse with 10 ml solution, two parameters were evaluated: 1) the salivary F concentration and 2) F retention in the oral cavity.
There was a clear dose–response effect with respect to the salivary F concentration of the three products. The results correspond well with those of our earlier studies, i.e. the higher the F concentration in the mouthrinse solution, the higher salivary F levels throughout the clearance period [16,17]. These data are also in agreement with another study, comparing 1–10 ml rinsing solution with 250–2500 ppm F [18], in which the authors concluded that‘the applied F con-centration is a more important factor than the applied F amount per se in determining the elevation of oral F levels following topical F use’. In the present study, a volume of 10 ml was selected, being the dosage recommended by most manufacturers and dental societies. However, if there is a risk that the patient might swallow too much of the solution, this volume could be reduced, for example, to 5 ml, probably without any loss of effect.
There may be several advantages of mouthrinsing with a F concentration as high as 1450 ppm, used either as a post-brushing rinse, or on a separate occasion between post-brushings. Mystikos et al. [14] found that a post-brushing mouthrinse solution with 900 ppm F might be suitable for patients at high risk for caries, especially for those who rinse extensively after brushing. For this group of patients – and in light of the results of the present study– a mouthrinse solution con-taining 1450 ppm F might be preferable even to 900 ppm F and certainly preferable to 225 ppm F.
With respect to availability of F in the oral cavity, rinsing solutions may have some advantages over a dentifrice or a brush-on gel [19]. Thus, a solution which is swished around the dentition for one minute with active cheek and lip move-ments, may easily penetrate the dental biofilm and also reach the approximal tooth surfaces. This is supported by an experimental in situ study, showing that F rinsing achieved
0 20 40 60 80 100 120 140 160 0 10 20 30 40 50 60 70 pp m fl u ori d e Minutes 0.05 % NaF 0.2 % NaF 0.32 % NaF (a) (b)
Figure 1. (a, b) Mean salivary fluoride concentration (non-logarithmic (a) and logarithmic (b)) as a function of time in expectorated saliva at 1, 3, 5, 10, 20, 30, 45 and 60 min after one minute’s single, supervised use of 10 ml of the indicated mouthrinse.
greater remineralization of demineralized enamel and den-tine specimens located approximally than buccally [20].
Recently, Parkinson et al. [21], using an in situ caries model, showed that brushing with a 1150 ppm F dentifrice, followed by a 225 ppm F mouthrinse, is effective in promot-ing remineralization of an enamel caries lesion. They con-cluded that‘F mouthrinses provide advantages for F delivery by maintaining elevated intra-oral F concentrations following F dentifrice use’. The specimens were flush with the surface of the buccal flange of the participant’s partial denture. It may be speculated that the additional effect of the rinsing solution would have been even more pronounced if a 900 or 1450 ppm F solution had been used instead of 225 ppm F and if the specimens had been sited approximally instead of buccally.
There are a substantial number of clinical studies showing the additional caries preventive effect of F rinsing to comple-ment daily use of F toothpaste [11,12]. For example, a 3-year randomized controlled trial of school-based F mouthrinsing (0.2% NaF) among 13- to 16-year-olds (n ¼ 622), using tooth-paste at home, was carried out in Sweden 1999–2003 [22]. The main conclusion was that rinsing with F at school reduces caries: the preventive effect varied between 30 and 59% [22]. Apart from clinical trials, there are alsoin situ stud-ies demonstrating the additional effect of F rinsing as an adjunct to F toothpaste for prevention of both dental
erosion [23] and caries [21]. In this context, a recent study, using micro-computed tomography, has shown that in com-bination with twice daily brushing with a NaF dentifrice, bed-time rinsing with a 0.05% NaF solution increased remineralization of an incipient caries-like lesionin situ [19].
Root caries is an increasing problem among elderly peo-ple and there is a need for simpeo-ple preventive measures. This has recently been discussed in a review by Magalh~aes [24]: ‘Among the self-applied products, there is strong evidence that 5000 ppm F toothpaste is more effective in arresting root caries lesions and in preventing new lesions compared to 1100–1450 ppm F toothpastes. Wierichs and Meyer-Lueckel [7], in a systematic review of non-invasive treatment of root caries lesions, support regular use of dentifrices con-taining 5000 ppm F. Both these authors however also point out that a previous review by Marinho et al. [11], revealed a lower coronal caries incidence associated with use of a F rinse in addition to brushing with a F dentifrice. Wierichs and Meyer-Lueckel [7] conclude, “since the findings for cor-onal caries showed a rather good efficacy, it seems plausible to recommend the daily use of NaF rinses to reduce not only the initiation of coronal caries lesions but also the initiation of root caries lesions”. Among the reviewed self-applied top-ical fluoride methods, daily use of a 0.2% sodium fluoride (NaF) mouth rinse is most likely to be the most effective’ [25]. Based on the literature reviews and the results of the present study, daily mouthrinsing with 900 ppm F, or prefer-ably with 1450 ppm F, as a supplement to daily use of F toothpaste, has potential as a preventive measure for elderly people at risk of root caries.
Beside dental caries, a high F solution may also be useful for prevention of dental erosion. In a recent review by Huysmans et al. [15], the authors discuss various strategies to prevent dental wear. Although stannous fluoride (SnF2) has received increasing attention as a promising anti-erosive agent, the use of high concentrations of NaF in dentifrices and mouthrinses is also discussed. To date, there are no long-term clinical studies on the effects of F solutions in pre-venting erosion of enamel and dentine. A high F solution such as the 0.32% NaF solution tested in the present study, may have potential application as a daily rinse in patients at risk of erosion. In an in situ erosion remineralization model, Maggio et al. [23] studied the combination of NaF toothpaste (1450 ppm F) and NaF mouthrinse (450 ppm F). There was a significant effect on hardening of incipient erosive lesions of enamel and increased resistance to a second erosive chal-lenge. In future studies it would be of interest to evaluate the effect of a 1450-ppm F rinse in this model.
Finally, with reference to the second aim of the present study, i.e. F retention in the oral cavity: increasing the F con-centration from 900 to 1450 ppm F results in a difference of the order of 1.61. This is in accordance with the results in Figure 3, showing that for the 0.32% NaF solution, mean F retention was 1.31 mg, compared to 0.86 mg F for the 0.2% NaF (1.31/0.86 1.52). The large range reflects individual var-iations in retention, especially for the 1450 ppm F solution. If the products are used twice daily, the estimated ingestion is around 2.6 mg and 1.7 mg F, respectively, which is half the
Figure 2. Mean values ± SD for AUC 3–60 min after rinsing with 0.05, 0.2 and 0.32% sodium fluoride (NaF) (p < .05, Tukey’s test).
Figure 3.Box-plot salivary fluoride retention ± SD after rinsing with 0.05, 0.2 and 0.32% sodium fluoride (NaF) (p < .05, two-tailed t-test).
recommended F intake from water and food (3–4 mg F/day) [26]. Thus, from a toxicological point of view, neither 0.2% NaF nor 0.32% NaF rinses are of concern.
A shortcoming of this study was that some saliva samples were too small to be analysed. However, most of the sam-ples were measurable and the statistical analysis disclosed significant inter-group differences.
In this study, standard procedure was followed, i.e. 10 ml solution was evaluated by expectorating once. Both the 900 and the 1450 ppm F rinsing solutions should be used only by children>12 years and by adults and elderly people who have no problem in spitting out the solution. There are sev-eral means of reducing F retention in the oral cavity and thereby the amount of F swallowed. One way is to reduce the volume of the solution, as mentioned above, from 10 ml to 5 ml. Alternatively instead of spitting out only once, spit-ting out twice in quick succession could be advised, or more vigorous spitting out.
For future research, it would be of interest to determine salivary F concentration and F retention after rinsing with 5 ml instead of 10 ml, in order to reduce F retention. Another topic to be investigated could be the above-mentioned parameters in subjects using the 0.32% mouthrinse as an adjunct to brushing with high F toothpaste (5000 ppm F).
In conclusion, the overall aim of the present investigation was to evaluate a mouthrinse with high NaF concentration, recently introduced to the Swedish market. It contains 0.32% (1450 ppm F) and is sold over-the-counter (OTC). The product was compared with two commonly used OTC products avail-able in many countries for daily or weekly rinsing, containing either 0.2% (900 ppm F) or 0.05% NaF (225 ppm F). The results disclosed a dose–response effect. The mouthrinse solution with 0.32% NaF gives both higher salivary F concen-trations over time and higher F retention than a 0.2% NaF solution and much higher retention than a 0.05% NaF solu-tion. It is concluded that this high F mouthrinse has potential benefit for prevention of caries and erosion.
Acknowledgements
The authors thank Per-Erik Isberg for the statistical analyses and Joan Carrick for the English revision.
Disclosure statement
The authors report no conflicts of interest. The authors alone are respon-sible for the content and writing of the paper.
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