Molecular Biotechnology Programme Uppsala University School of Engineering UPTEC X 01 010
Date of issue 2001-02Author
Erika Spens
Title (English)
In vitro profiles of human, rat and mouse fractalkine for rat and mouse chemokine receptor CX
3CR1
Title (Swedish)
Abstract
In the present study the binding affinities and the intrinsic activities of human, rat and mouse fractalkine (FKN) were investigated at the rat and mouse FKN receptor CX3CR1, both expressed in HEK-293 cells. The chemokine FKN possesses unique properties among other chemokines by its cystein motif (CX3C) and by being membrane bound. The extracellular part (FKN(l)) is comprised of a chemokine domain (FKN(s)) carried on top of a mucin-like stalk and it can be proteolytically cleaved to generate soluble FKN. The profiles of both FKN(l) and FKN(s) were investigated and the rank order of potency was determined to be human >
rat > mouse at both receptor types. Human and rat FKN(s) exhibited higher affinity than the corresponding FKN(l) while the opposite was true for mouse FKN.
Keywords
fractalkine, neurotactin, chemokine, CX3C, fractalkine receptor, CX3CR1, multiple sclerosis Supervisors
Åsa Malmberg and Maria Wanderoy
AstraZeneca R&D Södertälje, Sweden Examiner
Åsa Malmberg
AstraZeneca R&D Södertälje, Sweden
Project name Sponsors
Language
English Security
ISSN 1401-2138 Classification
Supplementary bibliographical information Pages
35
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