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Molecular Biotechnology Programme Uppsala University School of Engineering UPTEC X 01 023
Date of issue 2001-05Author
Niklas Henriksson
Title (English)
Modulating Poly(A)-specific ribonuclease (PARN) activity in vitro by RNA binding proteins
Title (Swedish)
Abstract
mRNA degradation is an important step in the regulation of gene expression. The first step in mRNA decay is removal of the poly(A)-tail in the 3’-end, carried out by the poly(A)-specific ribonuclease (PARN). However, the mRNA exists as a complex with RNA binding proteins when deadenylation is about to start. The aim of this study was to investigate how the RNA-binding proteins poly(A) binding protein (PABP) and p100 affect the activity of PARN. Recombinant PABP, p100 and PARN was expressed and purified. The RNA-binding capacity of PABP and p100 was investigated in vitro. PABP was found to inhibit the effect of PARN in vitro when low mRNA concentration (relative to PABP) was used. However, at high mRNA concentration, PABP showed both stimulatory and inhibitory effects on PARN depending on the specific concentrations provided. Therefore, the amount of PABP in the cytoplasm might be a way for the cell to control mRNA degradation.
Keywords
mRNA degradation, deadenylation, PARN, RNA binding proteins
Supervisors
Anders Virtanen
Department of Cell and Molecular Biology, Uppsala University Examiner
Leif Kirsebom
Department of Cell and Molecular Biology, Uppsala University
Project name Sponsors
Language
English
Security
ISSN 1401-2138 Classification
Supplementary bibliographical information Pages
18
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