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Molecular Biotechnology Programme

Uppsala University School of Engineering

UPTEC X 13 023 Date of issue 2014-01

Author

Johan Lagmo

Title (English)

Development of a multiplex real time PCR assay to target bacteria causing meningitis

Abstract

Meningitis is a severe disease with fatal outcome if not treated properly. This project evaluate and recommend a variation of multiplex qPCR assays which identify three bacteria causing meningitis, Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae.

The evaluation was based on the analytical sensitivity and specificity of the assays and a comparative analysis on clinical specimens. The qPCR assay was also prepared to be a routine method for the Academic Hospital in Uppsala.

Keywords

Meningitis, Real Time PCR, qPCR, multiplex PCR, Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, analytical sensitivity

Supervisors

Björn Herrmann

Uppsala University Scientific reviewer

Jonas Blomberg

Uppsala University

Project name Sponsors

Language

English

Security

ISSN 1401-2138 Classification

Supplementary bibliographical information Pages

39

Biology Education Centre Biomedical Center Husargatan 3 Uppsala

Box 592 S-75124 Uppsala Tel +46 (0)18 4710000 Fax +46 (0)18 471 4687

References

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