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Bioinformatics Program Uppsala University School of Engineering

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Bioinformatics Program

Uppsala University School of Engineering

UPTEC X 06 047 Date of issue 2006-12

Author

Linn Fagerberg

Title (English)

Bioinformatic analysis of human membrane proteins for antibody-based proteomics

Title (Swedish)

Abstract

Membrane proteins are important targets for the pharmaceutical industry and therefore in focus for antibody-based proteomics efforts such as the HPA program. In this project,

prediction methods for membrane protein topology have been assessed, and six methods were selected for implementation into an antigen selection software. A pilot study for validation of the selected methods was performed by flow cytometry using HPA antibodies.

Keywords

proteomics, antibody, membrane protein, topology prediction methods, flow cytometry Supervisors

Mathias Uhlén

School of Biotechnology, Royal Institute of Technology

Scientific reviewer

Erik Sonnhammer

Stockholm Bioinformatics Center

Project name Sponsors

Language

English

Security

ISSN 1401-2138 Classification

Supplementary bibliographical information Pages

38

Biology Education Centre Biomedical Center Husargatan 3 Uppsala Box 592 S-75124 Uppsala Tel +46 (0)18 4710000 Fax +46 (0)18 555217

References

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