Bioinformatics Engineering Program
Uppsala University School of Engineering
UPTEC X 15 007
Date of issue 2015-06 AuthorAnna Fogelström
Title (English)
DNA barcoding of freshwater fishes in Matang, Malaysia
Title (Swedish) Abstract
DNA barcoding is a fairly recently developed method for species identification at the molecular level, often using a short DNA sequence from the mitochondrial genome. In this study DNA barcoding was applied to establish a framework at University of Malaya for rapid and efficient identification of freshwater fish of Malaysia instead of relying on morphological techniques that require high level of taxonomical knowledge and field experience. The results, when applying the established framework, indicate that the interspecific divergences
calculated for our target group are clearly greater than intraspecific variations within the group, i.e. a barcode gap seems to exist. Indications are hence that cytochrome c oxidase subunit 1 sequences as barcodes could be effective tools for rapid identification of freshwater fishes and results could provide a good start for researchers at University of Malaya to start build a reference library of barcode sequences.
Keywords
DNA barcoding, freshwater fish, COI, coxI, BOLD, mitochondrial DNA, Supervisors
Amir F Merican University of Malaya Scientific reviewer
Mikael Thollesson Uppsala University
Project name Sponsors
Language
English
Security
ISSN 1401-2138 Classification
Supplementary bibliographical information Pages
53
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