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Molecular Biotechnology Program Uppsala University School of Engineering

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UPTEC  X  12  019 Date of issue 2012-06 Author

Cheuk Hin Lau

Title (English)

Picha pastoris expressing recombinant spider silk, is it possible?

Title (Swedish)

Abstract

Native spider silk is a versatile biomaterial that is stronger than steel, tougher than Kevlar and yet very flexible. This combined with recent research showing biocompatibility with cells gives the spider silk a wider range of applications such as for biomedical applications. A total of 5 different genes (A, B, C, D, E) were cloned into P. pastoris, with each gene containing 4RepCT coupled to different tags. The genes were ligated to expression vectors pGAPZαC, pGAPZαA and pPICZαA and were successfully transformed to P. pastoris. So far gene B has been successfully expressed and secreted by P. pastoris using the expression vector

pPICZαA.

Keywords

Pichia pastoris, Spider silk, Cloning, Protein expression, Purification, Protease assay

Supervisors

My Hedhammar & Mats Sandgren

Swedish University of Agriculture Sciences (SLU)

Scientific reviewer

Margareta Krabbe

Uppsala University

Project name Sponsors

Language

English

Security

Secret until 2014-12 ISSN 1401-2138 Classification

Supplementary bibliographical information Pages

48

Biology Education Centre Biomedical Center Husargatan 3 Uppsala Box 592 S-75124 Uppsala Tel +46 (0)18 4710000 Fax +46 (0)18 471 4687

Molecular Biotechnology Program

Uppsala University School of Engineering

References

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